Jones R M, Lamb J H, Lim C K
MRC Toxicology Unit, University of Leicester, UK.
Rapid Commun Mass Spectrom. 1995;9(10):921-3. doi: 10.1002/rcm.1290091011.
A method is described for the rapid analysis of mixtures of porphyrin methyl esters using laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF MS). Ions corresponding to [M+H]+ for each component have been observed without the need for added matrix. LDI-TOF MS has been applied to the analysis and characterization of porphyrin methyl esters derived from urine samples of patients suffering from various porphyrias. This technique has been shown to provide "fingerprint" profiles of the porphyrins present in each urine sample, characteristic of the porphyria, which compare favourably with those obtained with the established method of high-performance liquid chromatography. LDI-TOF MS is a rapid and reliable method for the screening of urine from patients suffering from porphyria.
描述了一种使用激光解吸/电离飞行时间质谱（LDI-TOF MS）快速分析卟啉甲酯混合物的方法。无需添加基质即可观察到对应于各组分[M+H]+的离子。LDI-TOF MS已应用于对患有各种卟啉症患者尿液样本中衍生的卟啉甲酯进行分析和表征。已证明该技术可提供每个尿液样本中存在的卟啉的“指纹”图谱，这些图谱具有卟啉症的特征，与通过既定的高效液相色谱法获得的图谱相比具有优势。LDI-TOF MS是一种快速且可靠的用于筛查卟啉症患者尿液的方法。
