Dairi T, Nakano T, Mizukami T, Aisaka K, Hasegawa M, Katsumata R
Tokyo Research Laboratories of Kyowa Hakko Kogyo Co., Ltd., Japan.
Biosci Biotechnol Biochem. 1995 Jul;59(7):1360-1. doi: 10.1271/bbb.59.1360.
By genomic Southern blot analysis, the DNA sequences homologous to the gene cluster responsible for biosynthesis of 6-demethyl-chlortetracycline in Streptomyces aureofaciens NRRL3203 were shown to be highly conserved in independent chlortetracycline- or tetracycline producing Streptomyces strains. By contrast, oxytetracycline-producing Streptomyces strains had no hybridization with the cluster DNA.
通过基因组Southern印迹分析表明，与金色链霉菌NRRL3203中负责6-去甲基金霉素生物合成的基因簇同源的DNA序列，在独立的金霉素或四环素生产链霉菌菌株中高度保守。相比之下，土霉素生产链霉菌菌株与该基因簇DNA没有杂交信号。
