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使用费里特仪器监测培养箱中的二氧化碳水平。

Using Fyrite to monitor incubator carbon dioxide levels.

作者信息

Johnson J E, Boone W R, Lee S T, Blackhurst D W

机构信息

Greenville Hospital System, Reproductive Endocrinology Associates, South Carolina 29603, USA.

出版信息

J Assist Reprod Genet. 1995 Feb;12(2):113-7. doi: 10.1007/BF02211379.

DOI:10.1007/BF02211379
PMID:7670268
Abstract

PURPOSE

The Fyrite analyzer (Bacharach, Incorporated) was evaluated to ascertain whether it would perform within the range specified by the manufacturer (+/- 0.5%). Fyrite analyzer samples were compared to control samples obtained using an infrared CO2 monitor (Ohmeda, Model Number 5200).

METHODS

Ten Fyrite analyzer measurements from each of three incubators were obtained using two different analyzers by three investigators for a total of 60 measurements per investigator. Twenty control measurements for each of the three incubators were obtained using an infrared monitor calibrated at the 5.0% CO2 level.

RESULTS

Fyrite analyzer values fell outside of an acceptable range (Control mean CO2 +/- 0.5%) for 7 of 60 (11.7%) observations for Incubator 1 and 4 of 60 (6.7%) observations for Incubator 2. (Incubator 3 had no values outside the range.) The Fyrite analyzers were significantly different from each other (P = 0.002). The three investigators (I) differed significantly from the control (I1 and I3, P < 0.001; I2, P = 0.02) as well as from each other (I1 vs I2, P < 0.001; I1 vs I3, P < 0.001; I2 vs I3, P < 0.016).

CONCLUSIONS

Use of an infrared monitor for incubator calibration and monthly CO2 checks in concert with daily use of the Fyrite as a monitoring tool only is the better method.

摘要

目的

对Fyrite分析仪(巴哈拉克公司)进行评估,以确定其是否能在制造商规定的范围内(±0.5%)运行。将Fyrite分析仪的样本与使用红外二氧化碳监测仪(奥美德,型号5200)获得的对照样本进行比较。

方法

三名研究人员使用两台不同的分析仪,从三个培养箱中各获取10次Fyrite分析仪测量值,每位研究人员共进行60次测量。使用在5.0%二氧化碳水平校准的红外监测仪,对三个培养箱中的每一个进行20次对照测量。

结果

培养箱1的60次观察中有7次(11.7%)、培养箱2的60次观察中有4次(6.7%)的Fyrite分析仪测量值超出了可接受范围(对照二氧化碳平均值±0.5%)。(培养箱3没有超出范围的值。)Fyrite分析仪之间存在显著差异(P = 0.002)。三名研究人员(I)与对照相比有显著差异(I1和I3,P < 0.001;I2,P = 0.02),彼此之间也有显著差异(I1与I2,P < 0.001;I1与I3,P < 0.001;I2与I3,P < 0.016)。

结论

更好的方法是使用红外监测仪进行培养箱校准和每月二氧化碳检查,并结合每天仅将Fyrite用作监测工具。

相似文献

1
Using Fyrite to monitor incubator carbon dioxide levels.使用费里特仪器监测培养箱中的二氧化碳水平。
J Assist Reprod Genet. 1995 Feb;12(2):113-7. doi: 10.1007/BF02211379.
2
Sources of variation in measurements of carbon dioxide levels inside incubators.培养箱内二氧化碳水平测量的变异来源。
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Am J Clin Pathol. 1964 Feb;41:163-6. doi: 10.1093/ajcp/41.2.163.
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Simple construction of a carbon dioxide incubator.二氧化碳培养箱的简易构造。
Tech Bull Regist Med Technol. 1962 Sep;32:156-9.
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A CARBON DIOXIDE INCUBATOR FOR THE CLINICAL MICROBIOLOGY LABORATORY.临床微生物实验室用二氧化碳培养箱
Am J Clin Pathol. 1963 Nov;40:495-9. doi: 10.1093/ajcp/40.5.495.
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引用本文的文献

1
Sources of variation in measurements of carbon dioxide levels inside incubators.培养箱内二氧化碳水平测量的变异来源。
J Assist Reprod Genet. 1996 Aug;13(7):606-8. doi: 10.1007/BF02066617.