来自骚扰锥蝽后肠的α-D-珠蛋白片段刺激克氏锥虫腺苷酸环化酶并促进循环后期锥虫发育。

An alpha D-globin fragment from Triatoma infestans hindgut stimulates Trypanosoma cruzi adenylyl cyclase and promotes metacyclogenesis.

作者信息

Fraidenraich D, Peña C, Isola E L, Lammel E M, Coso O, Añel A D, Baralle F, Torres H N, Flawia M M

机构信息

Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, CONICET, Buenos Aires, Argentina.

出版信息

Biol Res. 1993;26(1-2):279-83.

PMID:7670540

Abstract

A peptide from hindguts of the Triatoma infestans, the hematophagous Chagas' insect vector, activates adenylyl cyclase activity in Trypanosoma cruzi epimastigote membranes and stimulates the in vitro differentiation of epimastigotes (proliferative and non-infectious forms) to metacyclic trypomastigotes (non-proliferative and infectious forms). The peptide was purified from hindguts of insects fed two days before with chicken blood. After purification, the peptide showed upon SDS-PAGE a single band of about 10 kDa. The sequence for 20 residues of the amino terminus of this peptide was: H2N-Met-Leu-Thr-Ala-Glu-Asp-Lys-Lys-Leu-Ile-Gln-Gln-Ala-Trp-Glu-Lys-Ala- Ala-Ser-His. This sequence corresponds to the amino terminus of chicken alpha D-globin. A synthetic peptide with the sequence of the 40 amino acids corresponding to the amino terminus of alpha D-globin, also stimulated T. cruzi adenylyl cyclase activity and promoted metacyclogenesis.

摘要

来自吸血的恰加斯病昆虫媒介——骚扰锥蝽后肠的一种肽，可激活克氏锥虫前鞭毛体膜中的腺苷酸环化酶活性，并刺激前鞭毛体（增殖性和非感染性形式）体外分化为循环后期锥鞭毛虫（非增殖性和感染性形式）。该肽是从两天前喂食鸡血的昆虫后肠中纯化得到的。纯化后，该肽在SDS-PAGE上显示出一条约10 kDa的单一带。该肽氨基末端20个残基的序列为：H2N-Met-Leu-Thr-Ala-Glu-Asp-Lys-Lys-Leu-Ile-Gln-Gln-Ala-Trp-Glu-Lys-Ala-Ala-Ser-His。该序列对应于鸡αD-珠蛋白的氨基末端。一种具有与αD-珠蛋白氨基末端相对应的40个氨基酸序列的合成肽，也能刺激克氏锥虫腺苷酸环化酶活性并促进循环后期发育。