通过粒子枪将DNA导入海胆卵。
Introduction of DNA into sea urchin eggs by particle gun.
作者信息
Akasaka K, Nishimura A, Hijikata K, Iuchi Y, Morokuma J, Takahashi M, Morikawa H, Shimada H
机构信息
Graduate Department of Gene Science, Faculty of Science, Hiroshima University, Japan.
出版信息
Mol Mar Biol Biotechnol. 1995 Sep;4(3):255-61.
PMID:7670601
Abstract
We have applied the particle gun method, which was developed to introduce DNA into plant cells, to sea urchin eggs and have obtained excellent expression of the introduced DNA in the embryos. The expression can be normalized by concomitantly-introducing a reference construct. The method provides a new approach to quantitative analysis of cis-regulatory elements in sea urchin embryos.
摘要
我们已将用于将DNA导入植物细胞的粒子枪方法应用于海胆卵，并在胚胎中实现了导入DNA的出色表达。通过同时导入一个参照构建体，可使表达标准化。该方法为海胆胚胎中顺式调控元件的定量分析提供了一种新途径。
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