Sun P S
Molecular Endocrinology Program, Pacific Biomedical Research Center, University of Hawaii, Honolulu 96822, USA.
Mol Mar Biol Biotechnol. 1995 Sep;4(3):262-8.
Neuropeptides from the X-organ-sinus gland complex in the eyestalk have important roles in the regulation of growth and maturation in crustaceans. A complementary DNA encoding a molt-inhibiting hormone-like (MIH-like) neuropeptide from the eyestalk of Penaeus vannamei has been isolated and cloned in this laboratory. Using the reverse transcription-polymerase chain reaction (RT-PCR) method, the gene encoding the MIH-like neuropeptide was shown to be expressed in the brain as well as in the eyestalk. Subsequent cloning and sequencing of the PCR products showed that the MIH-like gene transcript of the brain shares 98% sequence identity with that of the eyestalk. In situ hybridization experiments showed that the MIH-like messenger RNA is exclusively localized in the X-organ complex in the medulla terminalis of the eyestalk, whereas in the brain the MIH-like gene transcript was detected in three regions including the neurosecretory cells, giant cells, and lateral cell bodies. These data suggest that the MIH-like peptide could have a specific function in nervous system activity other than its classic molt-inhibiting function.
来自对虾眼柄中X器官-窦腺复合体的神经肽在甲壳类动物的生长和成熟调节中发挥着重要作用。本实验室已从凡纳滨对虾的眼柄中分离并克隆出一种编码蜕皮抑制激素样（MIH样）神经肽的互补DNA。使用逆转录-聚合酶链反应（RT-PCR）方法，编码MIH样神经肽的基因在脑和眼柄中均有表达。随后对PCR产物进行克隆和测序表明，脑的MIH样基因转录本与眼柄的序列同一性为98%。原位杂交实验表明，MIH样信使核糖核酸仅定位在眼柄终髓的X器官复合体中，而在脑中，MIH样基因转录本在包括神经分泌细胞、巨细胞和外侧细胞体的三个区域中被检测到。这些数据表明，MIH样肽除了其经典的蜕皮抑制功能外，可能在神经系统活动中具有特定功能。
