Miková M, Grocholová R, Mardesic T, Zetová L, Müller P, Voboril J, Stroufová A
Ustav pro péci o matku a dítĕ, Praha.
Ceska Gynekol. 1995 Jun;60(3):145-9.
Two methods for embryo cryopreservation using 1,2-propanediol were compared--ultra-rapid vitrification and controlled freezing. No significant differences were found in the pregnancy and implantation rates although better results were achieved by the controlled freezing method. Significantly better results both in pregnancy and implantation rates were obtained when the embryos were frozen in the pronuclear stage compared to cleaved stage embryos. In the group of pronuclear stage embryos 3 pregnancies were achieved as a result of 11 embryo transfers (27%, implantation rate 9%) vs 14 pregnancies from 193 embryo transfers (7%, implantation rate 2.5%) for the cleaved embryos.
比较了两种使用1,2 - 丙二醇进行胚胎冷冻保存的方法——超快速玻璃化冷冻法和程序冷冻法。尽管程序冷冻法取得了更好的结果，但在妊娠率和着床率方面未发现显著差异。与分裂期胚胎相比，当胚胎在原核期冷冻时，妊娠率和着床率均显著提高。在原核期胚胎组中，11次胚胎移植中有3次妊娠成功（妊娠率27%，着床率9%），而分裂期胚胎193次胚胎移植中有14次妊娠成功（妊娠率7%，着床率2.5%）。
