通过一氧化碳生成量测定成年大鼠主动脉和肝脏中的血红素加氧酶活性。

Heme oxygenase activity in the adult rat aorta and liver as measured by carbon monoxide formation.

作者信息

Cook M N, Nakatsu K, Marks G S, McLaughlin B E, Vreman H J, Stevenson D K, Brien J F

机构信息

Department of Pharmacology and Toxicology, Faculty of Medicine, Queen's University, Kingston, ON, Canada.

出版信息

Can J Physiol Pharmacol. 1995 Apr;73(4):515-8. doi: 10.1139/y95-065.

DOI:10.1139/y95-065

PMID:7671194

Abstract

Rat aorta was homogenized and the 13000 x g supernatant fraction was tested for heme oxygenase (HO) activity by using a sensitive gas chromatographic method to measure carbon monoxide (CO), one of the products of the HO reaction. The rate of NADPH-dependent CO formation, an index of HO activity, was 1.41 +/- 0.40 nmol CO.mg(-1)protein. h(-1) in the rat aorta supernatant fraction and 2.05 +/- 0.55 nmol CO.mg(-1) protein.h(-1) in the rat liver 13000 x g supernatant fraction, a tissue known to contain HO activity. Chromium mesoporphyrin (0.05 mM), an inhibitor of rat liver HO, significantly decreased HO activity by 26% in the aorta supernatant fraction and 50% in the liver supernatant fraction. On the basis of the results of this study, which demonstrated HO-catalyzed CO formation in aortic tissue, and previous observations that CO relaxes vascular smooth muscle, we suggest that a physiological role for CO in vascular smooth muscle relaxation should be further investigated.

摘要

将大鼠主动脉匀浆，通过使用灵敏的气相色谱法测量血红素加氧酶（HO）反应产物之一一氧化碳（CO），来检测13000×g上清液部分的血红素加氧酶（HO）活性。作为HO活性指标的NADPH依赖性CO生成速率，在大鼠主动脉上清液部分为1.41±0.40 nmol CO·mg⁻¹蛋白质·h⁻¹，在已知含有HO活性的大鼠肝脏13000×g上清液部分为2.05±0.55 nmol CO·mg⁻¹蛋白质·h⁻¹。大鼠肝脏HO抑制剂中卟啉铬（0.05 mM）可使主动脉上清液部分的HO活性显著降低26%，使肝脏上清液部分的HO活性显著降低50%。基于本研究结果（该结果证明了主动脉组织中存在HO催化的CO生成）以及之前关于CO可使血管平滑肌舒张的观察结果，我们认为应进一步研究CO在血管平滑肌舒张中的生理作用。