Hashizoe M, Ogura Y, Takanashi T, Kunou N, Honda Y, Ikada Y
Department of Ophthalmology, Faculty of Medicine, Kyoto University, Japan.
Curr Eye Res. 1995 Jun;14(6):473-7. doi: 10.3109/02713689509003758.
We investigated the use of a scleral plug of biodegradable polymer implanted at the pars plana to create a controlled drug-delivery system in the vitreous. We evaluated the efficacy of a plug containing doxorubicin hydrochloride to treat experimental proliferative vitreoretinopathy (PVR) in pigmented rabbits. An implantable device on the sclera, which imitates a scleral plug, containing 1% doxorubicin, was prepared with poly(lactic acid) (molecular weight, 20,000). The release of doxorubicin in phosphate-buffered saline was evaluated by spectro-photometry. After pars plana vitrectomy and plug implantation, concentrations of doxorubicin in the vitreous humor of the rabbits were measured by high performance liquid chromatography. The release profiles were evaluated during 5 weeks in vitro and 4 weeks in vivo. Cultured homologous fibroblasts were injected into the vitreous space to induce experimental PVR after gas compression of the vitreous. The scleral plugs were implanted at the pars plana in treatment animals (n = 11). Control rabbits (n = 11) were followed up without implantation after PVR induction. All eyes of the control group developed tractional retinal detachment at day 28, while the incidence of retinal detachment was decreased to 64% in the treated eyes. (P = 0.002). The implantation of the scleral plug effectively inhibited intravitreous proliferation of fibroblasts. This study demonstrated that the scleral plug of biodegradable polymers may have potential as a treatment modality for PVR.
我们研究了在睫状体扁平部植入可生物降解聚合物巩膜塞以在玻璃体内创建可控药物递送系统的应用。我们评估了含盐酸多柔比星的巩膜塞治疗有色兔实验性增殖性玻璃体视网膜病变（PVR）的疗效。用聚乳酸（分子量20,000）制备了一种模仿巩膜塞的、含1%多柔比星的可植入巩膜装置。通过分光光度法评估多柔比星在磷酸盐缓冲盐水中的释放情况。在进行睫状体扁平部玻璃体切除和巩膜塞植入后，通过高效液相色谱法测量兔玻璃体液中多柔比星的浓度。在体外5周和体内4周期间评估释放曲线。在玻璃体气体压缩后，将培养的同源成纤维细胞注入玻璃体腔以诱导实验性PVR。在治疗组动物（n = 11）的睫状体扁平部植入巩膜塞。对照组兔（n = 11）在诱导PVR后不植入并进行随访。对照组所有眼在第28天均发生牵引性视网膜脱离，而治疗组眼中视网膜脱离的发生率降至64%。（P = 0.002）。巩膜塞的植入有效抑制了玻璃体内成纤维细胞的增殖。本研究表明，可生物降解聚合物巩膜塞可能具有作为PVR治疗方式的潜力。
