Le Beau J M, Liuzzi F J, Depto A S, Vinik A I
Department of Internal Medicine, Eastern Virginia Medical School, Norfolk, Virginia 23501, USA.
Exp Neurol. 1995 Jul;134(1):150-5. doi: 10.1006/exnr.1995.1045.
A recent study in our laboratory showed that B2 laminin gene is expressed by all L4 and L5 dorsal root ganglion (DRG) neurons as well as by satellite and Schwann cells. Because the laminin B2 subunit has a domain that supports neurite extension in culture, the present study was undertaken to test the hypothesis that laminin B2 gene expression would increase during sciatic nerve regeneration. In situ hybridization was used to examine B2 laminin gene expression in L4 and L5 DRGs 28 days after creating and bridging a 10-mm sciatic nerve gap with an impermeable silicone tube. Overall there was a nearly threefold increase in DRG B2 laminin chain mRNA at this timepoint, a time when axons are known to show vigorous regrowth. Both neurons and nonneuronal cells contributed to this increase. These data suggest that an up-regulation of B2 laminin gene expression by DRG neurons and nonneuronal cells may play a role in peripheral nerve regeneration.
我们实验室最近的一项研究表明，B2层粘连蛋白基因在所有L4和L5背根神经节（DRG）神经元以及卫星细胞和雪旺细胞中均有表达。由于层粘连蛋白B2亚基具有一个在培养中支持神经突延伸的结构域，因此开展了本研究以检验层粘连蛋白B2基因表达在坐骨神经再生过程中会增加这一假设。在使用不可渗透的硅胶管创建并桥接10毫米坐骨神经间隙28天后，采用原位杂交技术检测L4和L5背根神经节中的B2层粘连蛋白基因表达。总体而言，在这个时间点，背根神经节B2层粘连蛋白链mRNA增加了近三倍，而此时已知轴突会出现旺盛的再生。神经元和非神经元细胞都促成了这种增加。这些数据表明，背根神经节神经元和非神经元细胞对B2层粘连蛋白基因表达的上调可能在周围神经再生中发挥作用。
