Kannan K, Divers S G, Lurie A A, Chervenak R, Fukuda M, Holcombe R F
Department of Medicine, LSU Medical Center, Shreveport 71130-3932, USA.
Eur J Haematol. 1995 Sep;55(3):145-51. doi: 10.1111/j.1600-0609.1995.tb00242.x.
Platelets may become activated in vivo in a number of prethrombotic conditions including cancer. In the present study, cell surface expression of lysosome-associated membrane protein-2 (lamp2) and CD63 (lamp3) were examined by flow cytometry in 15 healthy volunteers and 5 patients with metastatic cancer to determine their utility as markers of in vivo platelet activation. Unstimulated platelets from controls had low levels of lamp2 expression, in contrast to cancer patients, who had significantly elevated levels (3.79 +/- 1.48% vs 33.9 +/- 5.6%). Upon stimulation with collagen, a greater than two-fold increase in the number of platelets expressing detectable levels of lamp2 was seen only among controls and not in cancer patients. Stimulation with collagen also resulted in a nearly two-fold increase in the proportion of platelets expressing CD63 in the control group, and a less than 1.5-fold increase in CD63 was seen in the patient group. The results suggest that cell surface lamp2 and CD63, like cell surface expression of GMP-140, may be good indicators of in vivo platelet activation and may be potentially useful in identifying patients with prethrombotic disorders.
血小板可能会在包括癌症在内的多种血栓前状态下在体内被激活。在本研究中，通过流式细胞术检测了15名健康志愿者和5名转移性癌症患者体内溶酶体相关膜蛋白2（lamp2）和CD63（lamp3）的细胞表面表达，以确定它们作为体内血小板激活标志物的效用。与癌症患者相比，对照组未受刺激的血小板lamp2表达水平较低，癌症患者的lamp2水平显著升高（3.79±1.48%对33.9±5.6%）。在用胶原蛋白刺激后，只有对照组中表达可检测水平lamp2的血小板数量增加了两倍多，而癌症患者中未出现这种情况。胶原蛋白刺激还导致对照组中表达CD63的血小板比例增加了近两倍，而患者组中CD63的增加不到1.5倍。结果表明，细胞表面lamp2和CD63与GMP-140的细胞表面表达一样，可能是体内血小板激活的良好指标，并且可能在识别血栓前疾病患者方面具有潜在用途。
