Shimada K, Takahashi M, Ikeda M, Tanzawa K
Biological Research Laboratories, Sankyo Co. Ltd., Tokyo, Japan.
FEBS Lett. 1995 Sep 4;371(2):140-4. doi: 10.1016/0014-5793(95)00886-e.
We report the cloning and sequencing of 5'-terminal region of a beta form of rat ECE-1 cDNA which is different only in its N-terminal amino-acid sequence to the cDNA we have cloned previously (alpha form [K. Shimada et al. (1994) J. Biol. Chem. 269, 18275-18278]). No significant difference was found in the specific activity and substrate specificity between the two isoforms. The expression level of ECE-1 alpha mRNA was higher than that of ECE-1 beta in various rat cells and tissues, suggesting that the physiologically important isoform is ECE-1 alpha. The present findings verified the presence of two forms of ECE-1 over many species, which are created probably through alternative splicing.
我们报道了大鼠ECE-1 cDNAβ形式5'-末端区域的克隆和测序，该形式与我们之前克隆的cDNA（α形式[K. Shimada等人（1994年）《生物化学杂志》269, 18275 - 18278]）仅在其N末端氨基酸序列上有所不同。在两种同工型之间未发现比活性和底物特异性有显著差异。在各种大鼠细胞和组织中，ECE-1α mRNA的表达水平高于ECE-1β，这表明生理上重要的同工型是ECE-1α。目前的研究结果证实了在许多物种中存在两种形式的ECE-1，它们可能是通过可变剪接产生的。
