Kacem K, Seylaz J, Issertial O, Aubineau P
Laboratoire de Recherches Cérébrovasculaires, UA 641 CNRS, Université Paris VII, Faculté de médecine Lariboisière-Saint Louis, France.
J Auton Nerv Syst. 1995 May 17;53(1):57-68. doi: 10.1016/0165-1838(94)00165-g.
The aim of the present study was to determine whether sympathectomy could influence the relative expression of two intermediate filament proteins, desmin and vimentin, two markers of differentiation, in arterial smooth muscle cells of the young rat. Newborn rats were treated with either repeated guanethidine or saline injections. Sections of the abdominal aorta, the femoral artery, the basilar and the middle cerebral arteries were processed simultaneously for immunofluorescence with monoclonal antibodies against desmin and vimentin and were then examined under either a conventional or a confocal laser-scanning microscope. In both cases, the mean optical density of the muscle layer staining was estimated by computerized image analysis. All artery sections from guanethidine-treated animals showed a significantly higher vimentin expression (108-119% of control values). Desmin expression was not significantly different except in the femoral artery after sympathectomy, where it was decreased by 6%. The relative increase of vimentin expression in sympathectomized blood vessels observed in the present study directly confirms previous morphometric and ultrastructural studies indicating that the sympathetic innervation of cerebral and peripheral blood vessels influences the phenotypic features of smooth muscle cells during post-natal development.
本研究的目的是确定交感神经切除术是否会影响幼年大鼠动脉平滑肌细胞中两种中间丝蛋白（结蛋白和波形蛋白，两种分化标志物）的相对表达。新生大鼠接受反复胍乙啶注射或生理盐水注射。同时对腹主动脉、股动脉、基底动脉和大脑中动脉切片进行处理，用抗结蛋白和波形蛋白的单克隆抗体进行免疫荧光检测，然后在传统显微镜或共聚焦激光扫描显微镜下观察。在这两种情况下，通过计算机图像分析估计肌层染色的平均光密度。来自胍乙啶处理动物的所有动脉切片均显示波形蛋白表达显著升高（为对照值的108 - 119%）。除交感神经切除术后的股动脉外，结蛋白表达无显著差异，在股动脉中结蛋白表达下降了6%。本研究中观察到的交感神经切除血管中波形蛋白表达的相对增加直接证实了先前的形态计量学和超微结构研究，表明脑和外周血管的交感神经支配在出生后发育过程中影响平滑肌细胞的表型特征。
