Yamamoto H, Hayes Y O, deSerres S, Chang J, Tabata T, Meyer A A
Department of Surgery, North Carolina Jaycee Burn Center, University of North Carolina School of Medicine, Chapel Hill, USA.
J Trauma. 1995 Aug;39(2):279-84. doi: 10.1097/00005373-199508000-00015.
We studied 75 BALB/c mice to examine the role of impaired immunoglobulin M (IgM) synthesis in the increased risk of bacterial infection after burn injury by investigating the kinetics of IgM synthesis to peptidoglycan polysaccharide (PGPS), a ubiquitous bacterial antigen. Splenocytes were isolated 1, 5, and 8 days postburn (PBD) and cultured with lipopolysaccharide for 5 days. Culture supernatant was collected and anti-PGPS IgM and total IgM levels were measured by ELISA. Total IgM-secreting cells were measured by ELISPOT assay. Total IgM and anti-PGPS IgM per IgM-secreting cell were calculated. On PBD 1, anti-PGPS IgM synthesis but not total IgM synthesis is increased in burned animals. By PBD 5, total IgM and anti-PGPS IgM synthesis in the burn group start to fall and by PBD 8, both are significantly decreased. The early increase in anti-PGPS IgM synthesis represents a positive response to bacterial challenge. However, the late nonspecific decrease in total IgM and anti-PGPS IgM synthesis suggests a potential mechanism for increased susceptibility to bacterial infection 5 to 10 days after burn injury.
我们研究了75只BALB/c小鼠，通过研究对肽聚糖多糖（PGPS，一种普遍存在的细菌抗原）的IgM合成动力学，来探讨免疫球蛋白M（IgM）合成受损在烧伤后细菌感染风险增加中所起的作用。在烧伤后（PBD）1天、5天和8天分离脾细胞，并用脂多糖培养5天。收集培养上清液，通过酶联免疫吸附测定（ELISA）测量抗PGPS IgM和总IgM水平。通过酶联免疫斑点分析（ELISPOT测定）测量总IgM分泌细胞。计算每个IgM分泌细胞的总IgM和抗PGPS IgM。在PBD 1时，烧伤动物中抗PGPS IgM合成增加，但总IgM合成未增加。到PBD 5时，烧伤组的总IgM和抗PGPS IgM合成开始下降，到PBD 8时，两者均显著下降。抗PGPS IgM合成的早期增加代表对细菌挑战的阳性反应。然而，总IgM和抗PGPS IgM合成后期的非特异性下降表明了烧伤后5至10天对细菌感染易感性增加的潜在机制。
