Tabata T, deSerres S, Meyer A A
Department of Surgery, University of North Carolina School of Medicine, Chapel Hill.
J Trauma. 1993 Oct;35(4):598-603; discussion 603-4. doi: 10.1097/00005373-199310000-00016.
BALB/c mice (ischemia: 31; controls: 15) were studied to investigate the effects of intestinal ischemia on antibody synthesis to peptidoglycan polysaccharide (PGPS), a ubiquitous bacterial antigen found in both gram-positive and gram-negative bacteria. The gut ischemia model was produced by placing a vessel loop around the superior mesenteric vessels for 45 minutes. All animals in the ischemia group had visible gut ischemia. Eighteen animals (58%) in the ischemia group survived to 24 hours and all experienced total recovery of gut viability. Single-cell suspensions of splenic lymphocytes were made. After 5 days of culture with lipopolysaccharide, anti-PGPS immunoglobulin concentrations in culture supernatants were measured by ELISA using high-titer BALB/c anti-PGPS serum as control. The synthesis of immunoglobulin by 10(5) lymphocytes was significantly increased in the ischemia group compared with the controls. These results represent the translocation of bacteria after intestinal ischemia, and this antibody response may be important in resistance to sepsis and multiple organ dysfunction attributed to bacterial translocation.
研究了BALB/c小鼠（缺血组：31只；对照组：15只），以调查肠道缺血对肽聚糖多糖（PGPS）抗体合成的影响，PGPS是一种在革兰氏阳性菌和革兰氏阴性菌中均存在的普遍细菌抗原。通过在肠系膜上血管周围放置血管环45分钟来建立肠道缺血模型。缺血组所有动物均出现明显的肠道缺血。缺血组18只动物（58%）存活至24小时，且所有动物肠道活力完全恢复。制备了脾淋巴细胞单细胞悬液。在用脂多糖培养5天后，使用高滴度BALB/c抗PGPS血清作为对照，通过ELISA测定培养上清液中的抗PGPS免疫球蛋白浓度。与对照组相比，缺血组10(5)淋巴细胞免疫球蛋白的合成显著增加。这些结果代表了肠道缺血后细菌的易位，并且这种抗体反应可能在抵抗因细菌易位导致的败血症和多器官功能障碍中起重要作用。
