Ahmed K, Tomiyoshi K, Fukazawa K, Inoue T, Murakami M, Kobayashi I, Endo K
Department of Nuclear Medicine, Gunma University School of Medicine.
Kaku Igaku. 1995 Jul;32(7):695-701.
TRAb Dade kit is a radioreceptor assay that detects autoantibodies in serum of patients with thyroid diseases which interfere with the binding of 125I labeled TSH to its receptor. In this paper we examined the basic and clinical usefulness of newly developed TRAb assay kits. The intra and inter assay coefficients of variation were 7.9% and 8.4%, respectively. There was no significant effect of temperature and incubation time on TRAb values. The influence of conjugated, unconjugated bilirubin, hemoglobin and turbidity was not so significant on TRAb values. TRAb values in serum of 68 untreated Graves' disease were above the normal range, but most (78%) treated Graves' patients who became euthyroid after receiving antithyroid drug, showed the normal range. In patients with chronic renal failure TRAb values were also within the normal range. A significant correlation (y = 1.0x-0.88, r = 0.95) was observed between this TRAb kit and TRAb Cosmic values in various thyroid conditions. This new assay for TRAb was simple, rapid and reproducible. The measurement of serum TRAb values was useful for the evaluation of physiological and pathological function and seemed to be helpful in the management of patients with thyroid disease.
TRAb Dade试剂盒是一种放射受体分析法，用于检测甲状腺疾病患者血清中的自身抗体，这些自身抗体可干扰125I标记的促甲状腺激素（TSH）与其受体的结合。在本文中，我们研究了新开发的TRAb检测试剂盒的基本特性和临床实用性。该试剂盒的批内和批间变异系数分别为7.9%和8.4%。温度和孵育时间对TRAb值无显著影响。结合胆红素、未结合胆红素、血红蛋白和浊度对TRAb值的影响也不显著。68例未经治疗的格雷夫斯病患者血清中的TRAb值高于正常范围，但大多数（78%）接受抗甲状腺药物治疗后甲状腺功能恢复正常的格雷夫斯病患者，其TRAb值显示在正常范围内。慢性肾功能衰竭患者的TRAb值也在正常范围内。在各种甲状腺疾病状态下，该TRAb试剂盒与TRAb Cosmic值之间观察到显著相关性（y = 1.0x - 0.88，r = 0.95）。这种新的TRAb检测方法简单、快速且可重复。血清TRAb值的测定有助于评估生理和病理功能，似乎对甲状腺疾病患者的管理有帮助。
