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Neprilysin: assay methods, purification, and characterization.

作者信息

Li C, Hersh L B

机构信息

Department of Biochemistry, College of Medicine, University of Kentucky, Lexington 40536, USA.

出版信息

Methods Enzymol. 1995;248:253-63. doi: 10.1016/0076-6879(95)48018-8.

DOI:10.1016/0076-6879(95)48018-8
PMID:7674925
Abstract
摘要

相似文献

1
Neprilysin: assay methods, purification, and characterization.中性内肽酶:检测方法、纯化及特性
Methods Enzymol. 1995;248:253-63. doi: 10.1016/0076-6879(95)48018-8.
2
Neuropeptidases: candidate enzymes and techniques for study.
Biochem Soc Trans. 1994 Feb;22(1):122-7. doi: 10.1042/bst0220122.
3
Positional effects in the neprilysin (neutral endopeptidase) reaction.中性内肽酶反应中的位置效应。
Arch Biochem Biophys. 1994 Jan;308(1):133-6. doi: 10.1006/abbi.1994.1019.
4
Purification of meprin from human kidney and its role in parathyroid hormone degradation.
Biol Chem Hoppe Seyler. 1994 Dec;375(12):821-4.
5
Molecular cloning and biochemical characterization of a new mouse testis soluble-zinc-metallopeptidase of the neprilysin family.一种新的小鼠睾丸中性肽链内切酶家族可溶性锌金属肽酶的分子克隆与生化特性分析
Biochem J. 2000 Apr 15;347(Pt 2):419-29. doi: 10.1042/0264-6021:3470419.
6
Purification and characterization of a neutral zinc endopeptidase secreted by Flavobacterium meningosepticum.脑膜炎败血黄杆菌分泌的一种中性锌内肽酶的纯化与特性分析
Arch Biochem Biophys. 1994 May 15;311(1):127-32. doi: 10.1006/abbi.1994.1217.
7
Cell-specific activity of neprilysin 2 isoforms and enzymic specificity compared with neprilysin.与中性内肽酶相比,中性内肽酶2亚型的细胞特异性活性及酶特异性
Biochem J. 2002 May 1;363(Pt 3):697-705. doi: 10.1042/0264-6021:3630697.
8
Mapping the active site of endothelin-converting enzyme-1 through subsite specificity and mutagenesis studies: a comparison with neprilysin.通过亚位点特异性和诱变研究绘制内皮素转化酶-1的活性位点:与中性肽链内切酶的比较。
Arch Biochem Biophys. 2002 Feb 15;398(2):240-8. doi: 10.1006/abbi.2001.2708.
9
Neutral endopeptidase 24.11: structure, inhibition, and experimental and clinical pharmacology.中性内肽酶24.11:结构、抑制作用以及实验与临床药理学
Pharmacol Rev. 1993 Mar;45(1):87-146.
10
Inhibitors of neprilysin: design, pharmacological and clinical applications.
Methods Enzymol. 1995;248:263-83. doi: 10.1016/0076-6879(95)48019-6.

引用本文的文献

1
Protection against vascular leak in neprilysin transgenic mice with complex overexpression pattern.具有复杂过表达模式的中性内肽酶转基因小鼠对血管渗漏的保护作用。
Transgenic Res. 2016 Dec;25(6):773-784. doi: 10.1007/s11248-016-9969-x. Epub 2016 Jul 1.
2
Reduction of amyloid-beta levels in mouse eye tissues by intra-vitreally delivered neprilysin.玻璃体内注射中性内肽酶可降低小鼠眼组织中β-淀粉样蛋白水平。
Exp Eye Res. 2015 Sep;138:134-44. doi: 10.1016/j.exer.2015.06.027. Epub 2015 Jul 2.
3
Mercury Reduces the Enzymatic Activity of Neprilysin in Differentiated SH-SY5Y Cells.
汞降低分化的SH-SY5Y细胞中中性内肽酶的酶活性。
Toxicol Sci. 2015 May;145(1):128-37. doi: 10.1093/toxsci/kfv037. Epub 2015 Feb 10.
4
PKCε promotes HuD-mediated neprilysin mRNA stability and enhances neprilysin-induced Aβ degradation in brain neurons.蛋白激酶Cε(PKCε)促进HuD介导的中性内肽酶(Neprilysin)mRNA稳定性,并增强中性内肽酶诱导的脑神经元中淀粉样β蛋白(Aβ)的降解。
PLoS One. 2014 May 21;9(5):e97756. doi: 10.1371/journal.pone.0097756. eCollection 2014.
5
Brain endogenous estrogen levels determine responses to estrogen replacement therapy via regulation of BACE1 and NEP in female Alzheimer's transgenic mice.脑内源性雌激素水平通过调节 BACE1 和 NEP 来决定雌性阿尔茨海默病转基因小鼠对雌激素替代疗法的反应。
Mol Neurobiol. 2013 Jun;47(3):857-67. doi: 10.1007/s12035-012-8377-3. Epub 2012 Nov 20.
6
Degradation of islet amyloid polypeptide by neprilysin.脑啡肽酶对胰岛淀粉样多肽的降解作用。
Diabetologia. 2012 Nov;55(11):2989-98. doi: 10.1007/s00125-012-2678-y. Epub 2012 Aug 17.
7
Active site mutations change the cleavage specificity of neprilysin.活性位点突变改变了 Neprilysin 的切割特异性。
PLoS One. 2012;7(2):e32343. doi: 10.1371/journal.pone.0032343. Epub 2012 Feb 23.
8
Genetic targeting aromatase in male amyloid precursor protein transgenic mice down-regulates beta-secretase (BACE1) and prevents Alzheimer-like pathology and cognitive impairment.在雄性淀粉样前体蛋白转基因小鼠中靶向遗传芳香酶可下调β-分泌酶(BACE1)并预防类似阿尔茨海默病的病理和认知障碍。
J Neurosci. 2010 May 26;30(21):7326-34. doi: 10.1523/JNEUROSCI.1180-10.2010.
9
Neprilysin null mice develop exaggerated pulmonary vascular remodeling in response to chronic hypoxia.中性内肽酶基因敲除小鼠在慢性低氧环境下会出现过度的肺血管重塑。
Am J Pathol. 2009 Mar;174(3):782-96. doi: 10.2353/ajpath.2009.080345.
10
Overexpression of neprilysin reduces alzheimer amyloid-beta42 (Abeta42)-induced neuron loss and intraneuronal Abeta42 deposits but causes a reduction in cAMP-responsive element-binding protein-mediated transcription, age-dependent axon pathology, and premature death in Drosophila.中性内肽酶的过表达可减少阿尔茨海默病β淀粉样蛋白42(Aβ42)诱导的神经元损失和神经元内Aβ42沉积,但会导致果蝇中cAMP反应元件结合蛋白介导的转录减少、年龄依赖性轴突病变和过早死亡。
J Biol Chem. 2008 Jul 4;283(27):19066-76. doi: 10.1074/jbc.M710509200. Epub 2008 May 7.