Whitaker N J, Bryan T M, Bonnefin P, Chang A C, Musgrove E A, Braithwaite A W, Reddel R R
Children's Medical Research Institute, Westmead, Sydney, Australia.
Oncogene. 1995 Sep 7;11(5):971-6.
Involvement of the retinoblastoma susceptibility (RB-1), p16INK4, p53 and telomerase genes in immortalisation was examined by determining their status in 15 human cell lines representing four immortalisation complementation groups. No abnormalities of RB-1, p53 and p16INK4 were detected in cell lines containing DNA tumour virus proteins known to bind to the protein products of the RB-1 and p53 genes. In contrast, in all other cell lines from each of the four groups either RB-1 was mutant or p16INK4 protein was undetectable and there were cell lines containing p53 mutations in three of the groups. Telomerase activity was detected in 12/15 lines, including some of the virally immortalised lines and in some lines from each group. Since none of these changes correlated with complementation group, other genetic changes must be required for immortalisation.
通过测定15个人类细胞系（代表四个永生化互补组）中视网膜母细胞瘤易感基因（RB - 1）、p16INK4、p53和端粒酶基因的状态，研究了这些基因在永生化过程中的作用。在含有已知可与RB - 1和p53基因的蛋白质产物结合的DNA肿瘤病毒蛋白的细胞系中，未检测到RB - 1、p53和p16INK4的异常。相反，在四个组中每组的所有其他细胞系中，要么RB - 1发生突变，要么检测不到p16INK4蛋白，并且在其中三个组中有含有p53突变的细胞系。在12/15个细胞系中检测到端粒酶活性，包括一些病毒永生化细胞系以及每组中的一些细胞系。由于这些变化均与互补组无关，因此永生化必定需要其他遗传变化。
