Jacob A, Kandpal G, Patanjali S R, Kandpal R P
Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
Oncogene. 1995 Sep 7;11(5):981-6.
We have used direct cDNA selection to isolate expressed sequences from a set of four overlapping YACs, spanning approximately 470 kb of the chromosomal interval 17q21 centromeric to BRCA1 gene. Thirty-eight nonoverlapping unique cDNA fragments were identified in this region. Twenty-two of the selected cDNAs showed complete identity with known genes and expressed sequence tags. Two of these cDNAs shared sequence homology with genes known to encode potential DNA binding motifs and hence could function in transcriptional regulation. The remaining 16 unique cDNA fragments showed no significant similarity to sequences in the databases and could potentially encode novel genes. Northern analysis of the novel cDNAs showed differential expression in various tissues, supporting the transcribed nature of these sequences. Our results place the gene for a G-protein coupled receptor (GPR2) previously mapped to 17q within a 400 kb region on 17q21.
我们利用直接cDNA筛选法从一组四个重叠的酵母人工染色体（YAC）中分离出表达序列，这些YAC跨越了从17q21着丝粒到BRCA1基因的大约470 kb染色体区间。在该区域鉴定出了38个不重叠的独特cDNA片段。其中22个选定的cDNA与已知基因和表达序列标签完全一致。这些cDNA中有两个与已知编码潜在DNA结合基序的基因具有序列同源性，因此可能在转录调控中发挥作用。其余16个独特的cDNA片段与数据库中的序列没有显著相似性，可能编码新基因。对新cDNA的Northern分析表明它们在各种组织中存在差异表达，支持了这些序列的转录性质。我们的结果将先前定位到17q的G蛋白偶联受体（GPR2）基因定位在17q21上的一个400 kb区域内。
