Rogers A H, Zilm P S
Microbiology Laboratory, Department of Dentistry, University of Adelaide, South Australia.
Oral Microbiol Immunol. 1995 Apr;10(2):119-21. doi: 10.1111/j.1399-302x.1995.tb00130.x.
Grown in a chemically defined medium containing glucose at a dilution rate of D = 0.065 h-1, Fusobacterium nucleatum D212B-2 produced large amounts of intracellular polyglucose. Aliquots of this culture were starved by anaerobic incubation at 37 degrees C and at various times, assayed for intracellular polyglucose content and viability. This protocol was repeated using cells grown under the same conditions in a chemically defined medium, a medium lacking carbohydrate and in which the organism produced no intracellular polyglucose. Both cultures had 50% survival time values of about 1.5 h and were not eliminated even after 32 h of starvation. It was, therefore concluded that starvation-survival is not influenced by intracellular polyglucose. Starvation-survival was also determined for cells grown in a chemically defined medium at D = 0.048 h-1 and D = 0.12 h-1. The faster-grown cells had a 50% survival time of 3.8 h but were completely eliminated after 8-16 h of starvation. In contrast, slower-grown cells had a 50% survival time of 1.5 h but were not completely eliminated after 32 h of starvation. This illustrates the importance of cell history and technique standardization in comparing the starvation-survival of different organisms.
具核梭杆菌D212B - 2在含有葡萄糖、稀释率为D = 0.065 h⁻¹的化学限定培养基中生长时，会产生大量细胞内多聚葡萄糖。取该培养物的等分试样，在37℃下进行厌氧培养使其饥饿，并在不同时间测定细胞内多聚葡萄糖含量和活力。使用在相同条件下在化学限定培养基、缺乏碳水化合物且该生物体不产生细胞内多聚葡萄糖的培养基中生长的细胞重复此方案。两种培养物的50%存活时间值均约为1.5小时，即使在饥饿32小时后也未被消除。因此得出结论，饥饿存活不受细胞内多聚葡萄糖的影响。还测定了在化学限定培养基中以D = 0.048 h⁻¹和D = 0.12 h⁻¹生长的细胞的饥饿存活情况。生长较快的细胞的50%存活时间为3.8小时，但在饥饿8 - 16小时后被完全消除。相比之下，生长较慢的细胞的50%存活时间为1.5小时，但在饥饿32小时后未被完全消除。这说明了细胞生长历程和技术标准化在比较不同生物体的饥饿存活情况时的重要性。
