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[绵羊发情同期化及随后早期妊娠期间血液学参数的动态变化]

[Dynamic changes in hematologic parameters in the blood of sheep during estrus synchronization and in the subsequent early pregnancy].

作者信息

Krajnicáková M, Bekeová E, Maracek I, Hendrichovský V

机构信息

Research Institute of Experimental Veterinary Medicine, Kosice, Slovak Republic.

出版信息

Vet Med (Praha). 1995 Jun;40(6):177-80.

PMID:7676575
Abstract

Observation of hematological indices which are a reflection of physiological processes in the individual phases of the reproductive cycle, presents one of the methods of controlling the homeostasis of the ewe's organism. This work focused on the observation of selected indices during synchronization treatment and in the first days of pregnancy The experiment included 10 conventionally bred animals. Blood was obtained from the v. jugularis into an oxalate mixture on days 0 (date of synchronization), 3, 7 and 14 of Agelin implantation, on the day of oestrus and days 7, 14, 17 and 34 of pregnancy. Erythrocyte counts were determined by the Bürker flask method, hemoglobin concentrations by spectrophotometry and packed cell volume (PCV) by the method of Janetzki. For leucocyte count determination the Bürker flask method with Turk's solution was used; the percentual proportion of leucocytes was stated by the leucogram. During synchronization treatment and pregnancy the mean erythrocyte counts ranged from 9.16 +/- 0.81 to 9.74 +/- 0.61 T/l and from 9.09 +/- 0.84 to 9.47 +/- 1.11 T/l, respectively. Hemoglobin concentrations oscillated between 10.71 +/- 1.02 and 11.83 +/- 1.01 g/l. PCV values reached between 32.44 +/- 2.18 and 39.33 +/- 2.73 l/l with a significant increase on day 14 of Agelin implantation and days 17 and 34 of pregnancy (P < 0.05). This dynamics of the red blood components points to the occurrence of functional-morphological changes in the sexual apparatus during oestrus and Agelin treatment. Through mediation by the vascular system a change in the mean values may also occur (Kresan et al., 1979).(ABSTRACT TRUNCATED AT 250 WORDS)

摘要

对血液学指标的观察反映了生殖周期各个阶段的生理过程,是控制母羊机体稳态的方法之一。本研究聚焦于同步化处理期间及怀孕初期选定指标的观察。实验纳入了10只传统饲养的动物。在(同步化处理的)第0天(同步化日期)、阿吉林植入后的第3天、第7天和第14天、发情当天以及怀孕的第7天、第14天、第17天和第34天,从颈静脉采集血液至草酸盐混合物中。红细胞计数采用伯克氏血细胞计数板法测定,血红蛋白浓度采用分光光度法测定,血细胞压积(PCV)采用亚内茨基法测定。白细胞计数测定采用含特克氏溶液的伯克氏血细胞计数板法;白细胞的百分比比例通过白细胞分类计数表示。在同步化处理和怀孕期间,平均红细胞计数分别为9.16±0.81至9.74±0.61万亿个/升和9.09±0.84至9.47±1.11万亿个/升。血红蛋白浓度在10.71±1.02至11.83±1.01克/升之间波动。PCV值在32.44±2.18至39.33±2.73升/升之间,在阿吉林植入后的第14天以及怀孕的第17天和第34天显著升高(P<0.05)。红细胞成分的这种动态变化表明在发情期和阿吉林处理期间性器官发生了功能形态学变化。通过血管系统的介导,平均值也可能发生变化(克雷桑等人,1979年)。(摘要截选至250词)

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