Wang J, Kearney K, Derby M, Wernette C M
Department of Chemistry, Auburn University, AL 36849-5312, USA.
Biochem Biophys Res Commun. 1995 Sep 14;214(2):723-9. doi: 10.1006/bbrc.1995.2345.
A mitochondrial type I-like, ATP-independent, DNA topoisomerase, isolated from highly purified yeast mitochondria, is genetically related to nuclear topoisomerase I. We found that the mitochondrial topoisomerase activity cannot be detected in yeast mitochondrial extracts prepared from strains in which the topoisomerase I gene (TOP1) is disrupted. Thus, the topoisomerase activity associated with mitochondria is dependent upon the expression of the nuclear topoisomerase I gene.
从高度纯化的酵母线粒体中分离出一种类似线粒体I型、不依赖ATP的DNA拓扑异构酶，它与细胞核拓扑异构酶I存在遗传关联。我们发现，在拓扑异构酶I基因（TOP1）被破坏的菌株所制备的酵母线粒体提取物中，无法检测到线粒体拓扑异构酶活性。因此，与线粒体相关的拓扑异构酶活性依赖于细胞核拓扑异构酶I基因的表达。
