Katrukha A G, Bogatcheva N V, Gusev N B
Russian Centre of Molecular Diagnostics and Medical Care, M.V. Lomonosov Moscow State University.
FEBS Lett. 1993 Jan 2;315(1):25-8. doi: 10.1016/0014-5793(93)81125-j.
Human cardiac troponin T has been isolated and its properties compared with those of rabbit skeletal and bovine cardiac troponin T. Seven monoclonal antibodies to troponin T have been obtained. Two antibodies cross-reacted with both cardiac and skeletal troponin T and recognized epitopes located between residues 98-177 of bovine cardiac troponin T. Five other antibodies were specific for cardiac troponin T and recognized antigenic determinants located between residues 180-258 of bovine cardiac troponin T. Localization of antigenic determinants in the central part of troponin T seems to be due to the high hydrophilicity and flexibility of this part of the molecule. The monoclonal antibodies thus obtained may be used for diagnosing various types of human heart diseases.
人心脏肌钙蛋白T已被分离出来，并将其特性与兔骨骼肌和牛心脏肌钙蛋白T的特性进行了比较。已获得七种针对肌钙蛋白T的单克隆抗体。两种抗体与心脏和骨骼肌肌钙蛋白T都发生交叉反应，并识别位于牛心脏肌钙蛋白T第98 - 177位残基之间的表位。另外五种抗体对心脏肌钙蛋白T具有特异性，并识别位于牛心脏肌钙蛋白T第180 - 258位残基之间的抗原决定簇。肌钙蛋白T中央部分抗原决定簇的定位似乎是由于该分子这一部分具有高亲水性和柔韧性。由此获得的单克隆抗体可用于诊断各种类型的人类心脏病。
