Shimizu N, Kita K, Masuya M, Nishii K, Matsuoka N, Morita N, Miwa H, Shirakawa S
Second Department of Internal Medicine, Mie University, School of Medicine, Tsu, Japan.
Exp Hematol. 1993 Jan;21(1):119-25.
Leukemic cells from a patient with chronic myelocytic leukemia (CML) basophilic crisis were examined in an in vitro clonogenic assay using recombinant human hematopoietic growth factors to elucidate the proliferative and differentiative behaviors. More than 90% of the leukemic cells showed the morphologic characteristics of basophils and were positive for CD11b and CD13. The phenotype of the leukemic cells was different from that of mast cells. In the clonogenic assay using various recombinant growth factors, the leukemic cells were responsive to interleukin-3 (IL-3) and granulocyte-macrophage colony-stimulating factor (GM-CSF), but not to granulocyte-CSF (G-CSF), erythropoietin (Epo), or IL-4. IL-5 showed synergistic effects on colony formations induced by both IL-3 and GM-CSF. Transcripts of the GM-CSF receptor alpha chain gene were detected in the leukemic cells, but transcripts of the IL-4 receptor gene were not. Furthermore, c-kit and IL-7 receptor genes were expressed in the leukemic cells. Our results suggest that the differentiation pathway of basophils is different from that of mast cells, even though the receptor gene for stem cell factor (c-kit) was expressed on the basophilic leukemic cells, as it was on mast cells.
利用重组人造血生长因子，通过体外克隆形成试验对一名慢性粒细胞白血病（CML）嗜碱性粒细胞危象患者的白血病细胞进行检测，以阐明其增殖和分化行为。超过90%的白血病细胞表现出嗜碱性粒细胞的形态特征，且CD11b和CD13呈阳性。白血病细胞的表型与肥大细胞不同。在使用各种重组生长因子的克隆形成试验中，白血病细胞对白介素-3（IL-3）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）有反应，但对粒细胞集落刺激因子（G-CSF）、促红细胞生成素（Epo）或IL-4无反应。IL-5对IL-3和GM-CSF诱导的集落形成具有协同作用。在白血病细胞中检测到GM-CSF受体α链基因的转录本，但未检测到IL-4受体基因的转录本。此外，白血病细胞中表达了c-kit和IL-7受体基因。我们的结果表明，嗜碱性粒细胞的分化途径与肥大细胞不同，尽管干细胞因子（c-kit）的受体基因在嗜碱性白血病细胞上表达，如同在肥大细胞上一样。
