Ohlin M, Sundqvist V A, Mach M, Wahren B, Borrebaeck C A
Department of Immunotechnology, Lund University, Sweden.
J Virol. 1993 Feb;67(2):703-10. doi: 10.1128/JVI.67.2.703-710.1993.
The humoral immune response to human cytomegalovirus (CMV) membrane glycoprotein gp58/116 (gB) has been studied by establishing cell lines producing specific human monoclonal antibodies. These cell lines were generated from peripheral blood lymphocytes obtained from a healthy carrier. Hybridomas producing gp58/116-specific antibodies were detected by reactivity to procaryotically expressed proteins containing the major neutralizing epitopes of this glycoprotein complex. One antibody, ITC88, which recognized an epitope located between amino acid residues 67 and 86 of gp116, potently neutralized the virus at 1 to 2 micrograms of immunoglobulin G per ml. Only four of the six human antibodies detecting the major neutralizing domain of gp58 neutralized the virus, and none of them required complement for activity. All antibodies that bound mature, processed gp58 recognized a conformational epitope involving sequences between residues 549 and 635. However, small differences existed between the antibodies in the actual minimal requirement for C- and N-terminal parts of this epitope. By peptide mapping with several of the antibodies, the epitope was shown to consist mainly of residues between amino acids 570 to 579 and 606 to 619. Despite the conformational nature of the epitope, the antibodies recognized both reduced and denatured native antigen. Presence of carbohydrates was not required for antigen binding of these gp58-specific human antibodies, but in at least one case, it greatly enhanced antigen recognition, indicating an importance of carbohydrate structures in some epitopes within the major neutralizing specificity of gp58.
通过建立产生特异性人单克隆抗体的细胞系,对人巨细胞病毒(CMV)膜糖蛋白gp58/116(gB)的体液免疫反应进行了研究。这些细胞系由从健康携带者获得的外周血淋巴细胞产生。通过与含有该糖蛋白复合物主要中和表位的原核表达蛋白的反应性,检测产生gp58/116特异性抗体的杂交瘤。一种名为ITC88的抗体识别gp116氨基酸残基67至86之间的一个表位,在每毫升1至2微克免疫球蛋白G时能有效中和病毒。检测gp