Sundqvist V A
J Virol Methods. 1982 Nov;5(3-4):219-27. doi: 10.1016/0166-0934(82)90012-x.
A direct ELISA was developed for determination of IgM antibody to varicella zoster virus (VZV). With this sensitive method VZV IgM antibodies were detected in all patients with a varicella and in 84% with a herpes zoster infection. All but one of 28 renal allograft recipients had previously had varicella. A primary infection was seen in the last patient, and reactivated infections in 11 of the others. The VZV IgM response seems to be specific since patients with a herpes simplex virus (HSV) infection and a heterotypic VZV IgG titer rise did not have detectable VZV IgM. An indirect enzyme-linked immunosorbent assay (ELISA) for detection of IgG antibodies has been used for serodiagnosis of VZV infections and to determine the immune status. After injection of zoster immune globulin, it was possible to measure passively transferred antibodies.
开发了一种直接酶联免疫吸附测定法（ELISA）用于检测水痘带状疱疹病毒（VZV）的IgM抗体。采用这种灵敏的方法，在所有水痘患者及84%的带状疱疹感染患者中检测到了VZV IgM抗体。28例肾移植受者中，除1例之外，其余均曾患过水痘。最后1例患者出现原发性感染，其他患者中有11例出现再激活感染。VZV IgM反应似乎具有特异性，因为单纯疱疹病毒（HSV）感染且异型VZV IgG滴度升高的患者未检测到可检测的VZV IgM。用于检测IgG抗体的间接酶联免疫吸附测定法（ELISA）已用于VZV感染的血清学诊断及确定免疫状态。注射带状疱疹免疫球蛋白后，能够检测到被动转移的抗体。
