多药耐药（mdr1）基因产物起ATP通道的作用。
The multidrug resistance (mdr1) gene product functions as an ATP channel.
作者信息
Abraham E H, Prat A G, Gerweck L, Seneveratne T, Arceci R J, Kramer R, Guidotti G, Cantiello H F
机构信息
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
出版信息
Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):312-6. doi: 10.1073/pnas.90.1.312.
Abstract
The multidrug resistance (mdr1) gene product, P-glycoprotein, is responsible for the ATP-dependent extrusion of a variety of compounds, including chemotherapeutic drugs, from cells. The data presented here show that cells with increased levels of the P-glycoprotein release ATP to the medium in proportion to the concentration of the protein in their plasma membrane. Furthermore, measurements of whole-cell and single-channel currents with patch-clamp electrodes indicate that the P-glycoprotein serves as an ATP-conducting channel in the plasma membrane. These findings suggest an unusual role for the P-glycoprotein.
摘要
多药耐药（mdr1）基因产物P-糖蛋白负责将包括化疗药物在内的多种化合物从细胞中进行ATP依赖的外排。此处呈现的数据表明，P-糖蛋白水平升高的细胞会向培养基中释放ATP，其释放量与质膜中该蛋白的浓度成正比。此外，用膜片钳电极对全细胞电流和单通道电流的测量表明，P-糖蛋白在质膜中充当ATP传导通道。这些发现提示了P-糖蛋白具有一种不同寻常的作用。
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