Fehér E, Fodor M, Szentpály O, Koncz E, Fehér J
I. Anatómiai Intézet, Semmelweis Orvostudományi Egyetem, Budapest.
Orv Hetil. 1993 Jan 24;134(4):183-6.
With the use of different antisera and Avidin-Biotin-Peroxidase technique, several immunopositive nerve fibers were observed in the wall of the gallbladder of the cat. A large amount of neuropeptide Y, substance P, somatostatin, vasoactive intestinal polypeptide immunoreactive nerve fibers were found in all layers. The most numerous of neuropeptide was the neuropeptide Y. Some immunoreactive nerve cell bodies (neuropeptide Y, substance P, somatostatin) were also observed in the muscle layers. Only a few calcitonin gene-related peptide positive were found in the wall. Cholecystokinin immunoreactive nerve fibers were observed very rarely and were located only in the muscle layers. Enkephalin positive nerve fibers were not found. Under the electron-microscope these immunoreactive nerve fibers were observed in a very close situation to the epithelial lining, to the smooth muscle cells. There were some immunoreactive nerve fibers around the smooth muscle cells of the arterioles and arteries. The distribution and the situation on the effector cells it is supposed that these neuropeptides might have important role in the motility, in the changes of the absorption and secretion of the gallbladder.
运用不同的抗血清和抗生物素蛋白-生物素-过氧化物酶技术，在猫的胆囊壁中观察到了几条免疫阳性神经纤维。在各层均发现了大量的神经肽Y、P物质、生长抑素、血管活性肠肽免疫反应性神经纤维。神经肽中数量最多的是神经肽Y。在肌层中还观察到了一些免疫反应性神经细胞体（神经肽Y、P物质、生长抑素）。在胆囊壁中仅发现少数降钙素基因相关肽阳性纤维。胆囊收缩素免疫反应性神经纤维极少被观察到，且仅位于肌层。未发现脑啡肽阳性神经纤维。在电子显微镜下，这些免疫反应性神经纤维紧密靠近上皮衬里和平滑肌细胞。在小动脉和动脉的平滑肌细胞周围也有一些免疫反应性神经纤维。基于其在效应细胞上的分布和位置，推测这些神经肽可能在胆囊的运动、吸收和分泌变化中起重要作用。
