Lew D, Fuseler J W
Louisiana State University Medical Center, Shreveport.
J Oral Maxillofac Surg. 1993 Feb;51(2):154-8. doi: 10.1016/s0278-2391(10)80014-x.
This study investigated the mitotic activity in the subdermally induced capsule and the epithelium surrounding tissue expanders maintained by pulsed expansions. Tissue expanders were placed into the fascial plane beneath the panniculus muscle of the rat and expanded sequentially. The volume in the expander was rapidly increased to a constant level and maintained for 48 hours; this was followed by a total decrease of volume for 48 hours. The procedure was repeated with volumes of 20, 30, and 40 mL. At the conclusion of each cycle of expander inflation, a group of rats was killed, and biopsy specimens of the induced capsule and epithelium were obtained. The developing capsule exhibited a significant increase in the mitotic index in the layers adjacent to the expander. The highest mitotic index was obtained with an inflation of 30 mL. These observations suggest that cellular proliferation, growth, and development of the extracellular matrix in the induced subdermal capsule can be sustained by pulsed volume changes in the expander.
本研究调查了皮下诱导形成的包膜以及通过脉冲扩张维持的组织扩张器周围上皮组织中的有丝分裂活性。将组织扩张器置于大鼠腹直肌下的筋膜平面，并依次进行扩张。扩张器内的体积迅速增加至恒定水平并维持48小时；随后体积在48小时内完全减小。分别以20、30和40 mL的体积重复该过程。在每次扩张器充气周期结束时，处死一组大鼠，并获取诱导包膜和上皮组织的活检标本。发育中的包膜在与扩张器相邻的层中显示出有丝分裂指数显著增加。在充气30 mL时获得最高有丝分裂指数。这些观察结果表明，扩张器中的脉冲体积变化可维持诱导皮下包膜中细胞增殖、生长以及细胞外基质的发育。
