偏钒酸铵对酵母细胞色素P-450的抑制作用。

Inhibition of yeast cytochrome P-450 by ammonium metavanadate.

作者信息

Del Carratore R, Morichetti E, Galli A, Galeotti C, Bronzetti G

机构信息

Istituto di Mutagenesi e Differenziamento, CNR, Pisa, Italy.

出版信息

Mutat Res. 1993 Mar;301(3):165-70. doi: 10.1016/0165-7992(93)90073-5.

DOI:10.1016/0165-7992(93)90073-5

PMID:7679198

Abstract

Incubation of diploid D7 strain cells of Saccharomyces cerevisiae (grown in 20% glucose) in the presence of ammonium metavanadate (AMV) led to a decrease in the cytochrome P-450-dependent monooxygenase system (cytochrome P-450 level and 7-ethoxycoumarin O-deethylase). The electrophoretic analysis of microsomal fractions of yeast cells treated with metavanadate revealed a decrease in the intensity of the bands corresponding to a M(r) in the range of 51,000-58,000 Da compared with those observed in controls, i.e., cells grown in 20% glucose. Analysis of the cytochrome P-450 transcript showed that AMV treatment reduced the mRNA level. Our results suggest that AMV inhibits the yeast cytochrome P-450 system by acting at both the pre- and post-transcriptional levels.

摘要

在偏钒酸铵（AMV）存在的情况下，对酿酒酵母二倍体D7菌株细胞（在20%葡萄糖中生长）进行培养，导致细胞色素P-450依赖的单加氧酶系统（细胞色素P-450水平和7-乙氧基香豆素O-脱乙基酶）降低。对用偏钒酸盐处理的酵母细胞微粒体部分进行的电泳分析显示，与对照（即在20%葡萄糖中生长的细胞）相比，对应于51,000-58,000 Da范围内相对分子质量（M(r)）的条带强度降低。细胞色素P-450转录本分析表明，AMV处理降低了mRNA水平。我们的结果表明，AMV通过在转录前和转录后水平发挥作用来抑制酵母细胞色素P-450系统。