Henley J M, Jenkins R, Hunt S P
Department of Pharmacology, Medical School, University of Birmingham, Edgbaston, UK.
Neuropharmacology. 1993 Jan;32(1):37-41. doi: 10.1016/0028-3908(93)90127-o.
The autoradiographic distribution of binding sites for [3H]AMPA, [3H]kainate, [3H]MK-801 and [3H]L-glutamate and the in situ hybridization of GluR-A, GluR-B, GluR-C and GluR-D in rat spinal cord sections were determined. All of the radioligands had similar localisations with the dorsal horn being the most intensely labelled region. In the in situ hybridization experiments only the GluR-B mRNA was detected at high levels in the spinal cord with predominant expression in the substantia gelatinosa. These results support electrophysiological evidence for the presence of glutamate activated neurones in the spinal cord and suggest that these excitatory pathways are confined mainly to the dorsal horn.
测定了大鼠脊髓切片中[3H]AMPA、[3H]海人藻酸、[3H]MK-801和[3H]L-谷氨酸结合位点的放射自显影分布以及GluR-A、GluR-B、GluR-C和GluR-D的原位杂交情况。所有放射性配体的定位相似，背角是标记最强烈的区域。在原位杂交实验中，仅在脊髓中高水平检测到GluR-B mRNA，且主要在胶状质中表达。这些结果支持了脊髓中存在谷氨酸激活神经元的电生理证据，并表明这些兴奋性通路主要局限于背角。
