Reisdorf P, Maarse A C, Daignan-Fornier B
Laboratoire de Génétique Moléculaire, Université Paris-Sud, Orsay, France.
Curr Genet. 1993 Feb;23(2):181-3. doi: 10.1007/BF00352019.
In order to facilitate the process of epitope-tagging of yeast proteins, we have constructed two Saccharomyces cerevisiae-Escherichia coli shuttle vectors that allow fusion of a sequence encoding an epitope of the human c-myc protein at the 3' end of any gene. An example of the use of this technique is presented.
为了促进酵母蛋白表位标记的过程,我们构建了两种酿酒酵母-大肠杆菌穿梭载体,它们可使编码人c-myc蛋白表位的序列在任何基因的3'端融合。本文给出了该技术的使用示例。