Kefalides N A, Ohno N, Wilson C B
Connective Tissue Research Institute, University of Pennsylvania, Philadelphia.
Kidney Int. 1993 Jan;43(1):85-93. doi: 10.1038/ki.1993.15.
Sera from patients with antiglomerular basement membrane (anti-GBM) antibodies associated with Goodpasture syndrome (GP) or glomerulonephritis were tested by ELISA and electroimmunoblot against whole basement membrane collagen (type IV) isolated from bovine anterior lens capsule (ALC) and bacterial collagenase resistant domains of the collagen molecule, that is, the NC-1 and 7-S domains isolated from either ALC or bovine and human glomerular basement membrane (GBM). Reactivity was high with the NC-1 domain by both the ELISA and the electroimmunoblot techniques. Some of the anti-GBM sera reacted with both the NC-1 and 7-S domains of both human and bovine type IV collagen. At a time when the patients' sera reacted weakly with a collagenase digest of human GBM using a radioimmunoassay, the reactivity with the NC-1 domain was also low, but some of the sera continued to react with the 7-S domain. The data suggest that there may be heterogeneity in the nature of autoantibodies with respect to collagen type IV domain reactivity in the sera of patients with anti-GBM antibody disease.
采用酶联免疫吸附测定（ELISA）和电免疫印迹法，对患有与肺出血肾炎综合征（GP）或肾小球肾炎相关的抗肾小球基底膜（anti-GBM）抗体的患者血清，检测其针对从牛晶状体前囊（ALC）分离的全基底膜胶原蛋白（IV型）以及胶原蛋白分子中抗细菌胶原酶的结构域，即从ALC或牛和人肾小球基底膜（GBM）分离的NC-1和7-S结构域的反应性。通过ELISA和电免疫印迹技术，与NC-1结构域的反应性都很高。一些抗GBM血清与人及牛IV型胶原的NC-1和7-S结构域均发生反应。当患者血清使用放射免疫测定法与人GBM的胶原酶消化物反应较弱时，与NC-1结构域的反应性也较低，但一些血清继续与7-S结构域发生反应。数据表明，在抗GBM抗体疾病患者血清中，自身抗体针对IV型胶原结构域的反应性性质可能存在异质性。
