Migliorini P, Betschart B, Corradin G
Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
Eur J Immunol. 1993 Feb;23(2):582-5. doi: 10.1002/eji.1830230245.
The immunogenicity of the non-repetitive sequences of the Plasmodium berghei circumsporozoite (CS) protein was studied using synthetic peptides. Two CS sequences (residues 20-39 and 57-70) exhibiting T cell helper activity were identified. Immunization of BALB/c mice with a branched peptide containing either the 20-39 or the 57-70 sequence and two repeats (B epitope) in a linear sequence induced high titers of anti-repeat and anti-sporozoite antibodies. Mice immunized with the T-B construct (high antibody titers) or with the 57-70 epitope alone (no serum anti-repeat or anti-peptide antibodies) were protected to a similar degree after challenge with infective sporozoites. No protection was obtained in mice immunized with the 20-39 epitope. These results indicate that BALB/c mice can be protected either by effector T cells or by high levels of anti-repeat antibodies. Thus, in the same strain, a double mechanism of protection can be obtained by a synthetic peptide vaccine.
利用合成肽研究了伯氏疟原虫环子孢子（CS）蛋白非重复序列的免疫原性。鉴定出两个具有T细胞辅助活性的CS序列（第20 - 39位氨基酸残基和第57 - 70位氨基酸残基）。用含有20 - 39序列或57 - 70序列且在一条线性序列中有两个重复（B表位）的分支肽免疫BALB/c小鼠，可诱导产生高滴度的抗重复和抗子孢子抗体。用T - B构建体（高抗体滴度）或仅用57 - 70表位（无血清抗重复或抗肽抗体）免疫的小鼠，在用感染性子孢子攻击后受到相似程度的保护。用20 - 39表位免疫的小鼠未获得保护。这些结果表明，BALB/c小鼠可通过效应T细胞或高水平的抗重复抗体得到保护。因此，在同一品系中，合成肽疫苗可获得双重保护机制。
