一种新型核小体组装程序（果胶助力）。

A novel nucleosome assembly procedure (with a little help from pectin).

作者信息

Sobolewski C H, Klump H H, Lindsey G G

机构信息

Department of Biochemistry, University of Cape Town, Republic of South Africa.

出版信息

FEBS Lett. 1993 Feb 22;318(1):27-9. doi: 10.1016/0014-5793(93)81320-y.

DOI:10.1016/0014-5793(93)81320-y

PMID:7679654

Abstract

The acidic polysaccharide pectin (alpha-1,4-polygalacturonic acid) has been introduced as a nucleosome assembly facilitator as a substitute for polyglutamic acid. The pectin-assembled nucleosomes were indistinguishable from polyglutamic acid-assembled nucleosomes by thermal denaturation and DNAse I digestion. Pectin had two major advantages over polyglutamic acid-the yield of assembled cores was approximately 50% higher and the pectin could be easily removed after completion of the reassembly procedure by dialysis following pectinase cleavage.

摘要

酸性多糖果胶（α-1,4-聚半乳糖醛酸）已被引入作为核小体组装促进剂，以替代聚谷氨酸。通过热变性和DNA酶I消化，果胶组装的核小体与聚谷氨酸组装的核小体没有区别。果胶相对于聚谷氨酸有两个主要优点——组装核心的产量大约高50%，并且在重新组装过程完成后，通过果胶酶切割后透析可以很容易地去除果胶。