Onoue H, Kaito N, Tokudome S, Abe T, Tashibu K, Nagashima H, Nakamura N
Department of Neurosurgery, Jikei University School of Medicine, Tokyo, Japan.
J Cereb Blood Flow Metab. 1993 Mar;13(2):346-9. doi: 10.1038/jcbfm.1993.44.
This study demonstrated the time-dependent changes in postmortem responses of isolated human middle cerebral artery strips to vasodilators. The relaxation induced by prostaglandin (PG) I2 or nitroglycerin remained stable for 24 h postmortem. In arterial strips precontracted with PGF2 alpha, substance P and bradykinin both elicited relaxation that was almost completely abolished by removal of the endothelium. The endothelium-dependent response to both peptides was significantly degraded in strips obtained > 12 h postmortem. These results indicate a selective functional or anatomical vulnerability of the vascular endothelium compared with that of the vasodilator mechanisms of the smooth muscle in the postmortem period. However, cerebral arteries isolated from human cadavers within 12 h postmortem should be adequate for studies of both smooth muscle and endothelial reactivity to vasodilators.
本研究证明了离体人脑中动脉条对血管扩张剂的死后反应随时间的变化。前列腺素（PG）I2或硝酸甘油诱导的舒张在死后24小时内保持稳定。在预先用前列腺素F2α预收缩的动脉条中，P物质和缓激肽均引起舒张，去除内皮后这种舒张几乎完全消失。在死后超过12小时获得的条带中，对这两种肽的内皮依赖性反应显著降低。这些结果表明，与死后平滑肌的血管舒张机制相比，血管内皮在功能或解剖学上具有选择性易损性。然而，在死后12小时内从人体尸体分离的脑动脉应该足以用于研究平滑肌和内皮对血管扩张剂的反应性。
