Park C S, Lee S M, Uh S T, Kim H T, Chung Y T, Kim Y H, Choi B W, Hue S H, Lee H B
Department of Internal Medicine, Soonchunhyang University Hospital, Seoul, Korea.
J Allergy Clin Immunol. 1993 Feb;91(2):623-33. doi: 10.1016/0091-6749(93)90268-k.
To elucidate the role of T-cell activation in the physiologic changes and cellular infiltrations in the bronchial tree of patients with symptomatic bronchial asthma, we measured the concentration of soluble interleukin-2 receptor (sIL-2R), cellular compositions and IL-2R expression on cells in bronchoalveolar lavage (BAL) fluid. We also measured physiologic parameters, including a provocation concentration of histamine required to reduce the forced expiratory volume in 1 second by 20% of the prechallenge baseline (PC20).
The concentration of sIL-2R and the ratio of IL-2R expression on CD3 positive lymphocytes were significantly higher in BAL fluid from 20 patients with symptomatic bronchial asthma than concentrations in fluid from nine healthy controls (p < 0.01) and six asymptomatic patients with bronchial asthma (p < 0.05). The concentration of sIL-2R correlated with the percentage of IL-2R expression on CD3 positive lymphocytes (rs = 0.709, p < 0.01). There was no difference in sIL-2R concentration of BAL fluid between the healthy control and the patient with asymptomatic bronchial asthma. Among the inflammatory cells recovered in BAL fluid, the percentages of eosinophils showed significant positive correlation with the concentration of sIL-2R in BAL fluid in the patients with bronchial asthma. The concentration of sIL-2R significantly correlated with forced expiratory volume one second, peak expiratory flow rate, and PC20 when observed in all study subjects, but not in the patients with bronchial asthma.
We conclude that T-cell activation within the bronchial tree appears to have a role in the infiltration of eosinophils into the bronchial tree, which may lead to enhanced bronchial hyperreactivity in patients with bronchial asthma.
为了阐明T细胞激活在有症状支气管哮喘患者支气管树的生理变化和细胞浸润中的作用，我们测量了支气管肺泡灌洗（BAL）液中可溶性白细胞介素-2受体（sIL-2R）的浓度、细胞组成以及细胞上IL-2R的表达。我们还测量了生理参数，包括使一秒用力呼气量较激发前基线降低20%所需的组胺激发浓度（PC20）。
20例有症状支气管哮喘患者的BAL液中sIL-2R浓度以及CD3阳性淋巴细胞上IL-2R的表达比例显著高于9例健康对照者的BAL液浓度（p<0.01）以及6例无症状支气管哮喘患者的BAL液浓度（p<0.05）。sIL-2R浓度与CD3阳性淋巴细胞上IL-2R的表达百分比相关（rs = 0.709，p<0.01）。健康对照者和无症状支气管哮喘患者的BAL液中sIL-2R浓度无差异。在BAL液中回收的炎性细胞中，支气管哮喘患者的嗜酸性粒细胞百分比与BAL液中sIL-2R浓度呈显著正相关。在所有研究对象中观察时，sIL-2R浓度与一秒用力呼气量、呼气峰值流速和PC20显著相关，但在支气管哮喘患者中无此相关性。
我们得出结论，支气管树内的T细胞激活似乎在嗜酸性粒细胞浸润支气管树中起作用，这可能导致支气管哮喘患者的支气管高反应性增强。
