Izuhara K, Miyajima A, Harada N
Department of Immunology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304-1104.
Biochem Biophys Res Commun. 1993 Feb 15;190(3):992-1000. doi: 10.1006/bbrc.1993.1147.
Transfectants expressing the chimeric receptor which has human interleukin-4 receptor (hIL-4R) in the extracellular domain and human interleukin-2 receptor beta chain (hIL-2R beta) in the cytoplasmic domain showed continuous growth in response to human interleukin-4 (hIL-4), whereas transfectants expressing hIL-4R responded only transiently to hIL-4. The pattern of tyrosine phosphorylation induced by hIL-4 in transfectants expressing the chimeric receptor was very similar to that in transfectants expressing hIL-2R beta, but different from that in transfectants expressing hIL-4R, indicating that the chimeric receptor transduces the interleukin-2 like growth signal by hIL-4.
在细胞外区域具有人白细胞介素-4受体（hIL-4R）且在细胞质区域具有人白细胞介素-2受体β链（hIL-2Rβ）的嵌合受体表达转染子，在人白细胞介素-4（hIL-4）刺激下呈现持续生长，而表达hIL-4R的转染子对hIL-4仅产生短暂反应。hIL-4在表达嵌合受体的转染子中诱导的酪氨酸磷酸化模式，与在表达hIL-2Rβ的转染子中非常相似，但与在表达hIL-4R的转染子中不同，这表明嵌合受体通过hIL-4转导类似白细胞介素-2的生长信号。
