Castillo G M, Templeton D M
University of Toronto, Department of Clinical Biochemistry, Canada.
FEBS Lett. 1993 Mar 8;318(3):292-6. doi: 10.1016/0014-5793(93)80531-x.
Two chondroitin sulfate-containing complexes have been isolated from fetal bovine serum and shown to contain the serine protease inhibitor bikunin. A complex of 126 kDa contains bikunin linked by a chondroitin sulfate chain to a protein with homology to the HC2 component of the human inter-alpha-trypsin inhibitor. This complex represents the extracellular matrix stabilizing factor recently described as a bikunin-containing fraction necessary for expansion of the cumulus matrix [(1992) J. Biol. Chem. 267, 12380-12386]. A second complex of 236 kDa contains, in addition to bikunin and HC2, a bovine homolog of HC3 of the human pre-alpha-trypsin inhibitor. Thus, bovine bikunin is a chondroitin sulfate proteoglycan that achieves multifunctionality by linkage to proteins homologous to human serine antiproteinase complexes.
已从胎牛血清中分离出两种含硫酸软骨素的复合物，并证明它们含有丝氨酸蛋白酶抑制剂比基尼。一种126 kDa的复合物含有通过硫酸软骨素链与一种与人α-胰蛋白酶抑制剂HC2成分具有同源性的蛋白质相连的比基尼。这种复合物代表了最近被描述为卵丘基质扩张所必需的含比基尼部分的细胞外基质稳定因子[(1992) J. Biol. Chem. 267, 12380 - 12386]。第二种236 kDa的复合物除了含有比基尼和HC2外，还含有人类前α-胰蛋白酶抑制剂HC3的牛同源物。因此，牛比基尼是一种硫酸软骨素蛋白聚糖，通过与人类丝氨酸抗蛋白酶复合物的同源蛋白质相连而实现多功能性。
