Lorrain J, Angel I, Eon M T, Oblin A, Langer S Z
Synthélabo Recherche (L.E.R.S.) Bagneux, France.
Pharmacology. 1993;46(2):109-14. doi: 10.1159/000139035.
Exogenous galanin (9 pmol/min during 20 min i.a.) decreased insulin levels in the plasma sampled from the pancreatic vein of the blood-perfused pancreas of dogs in which ganglionic transmission and beta-adrenoceptors had been blocked. This effect was not modified by idazoxan (1 mg/kg followed by 0.02 mg/kg/min i.v.) but was partially reduced (50%) by glibenclamide (1 mg/kg followed by 0.02 mg/kg/min i.v.). This dose of glibenclamide blocked entirely the hypoinsulinemic activity of diazoxide, an activator of pancreatic ATP-modulated K+ channels, whereas the dose of idazoxan prevented the effect of the alpha 2-adrenoceptor agonist UK-14,304. Therefore, in dogs, the decrease in insulin secretion produced by exogenous galanin is only partially mediated by activation of glibenclamide-sensitive ATP-gated K+ channels and is independent of alpha 2-adrenoceptor stimulation.
在外源性甘丙肽（20分钟内以9皮摩尔/分钟的速度进行胰腺内注射）作用下，从神经节传递和β-肾上腺素能受体已被阻断的犬的血液灌注胰腺的胰腺静脉采集的血浆中，胰岛素水平降低。伊达唑啉（1毫克/千克静脉注射，随后以0.02毫克/千克/分钟的速度静脉注射）不会改变这种作用，但格列本脲（1毫克/千克静脉注射，随后以0.02毫克/千克/分钟的速度静脉注射）可使其部分减弱（50%）。该剂量的格列本脲完全阻断了二氮嗪（一种胰腺ATP调节钾通道激活剂）的降胰岛素活性，而伊达唑啉剂量则可阻止α2-肾上腺素能受体激动剂UK-14,304的作用。因此，在犬中，外源性甘丙肽引起的胰岛素分泌减少仅部分由格列本脲敏感的ATP门控钾通道激活介导，且与α2-肾上腺素能受体刺激无关。
