Weistrand C, Norén K
Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden.
J Chromatogr. 1993 Feb 5;630(1-2):179-86. doi: 10.1016/0021-9673(93)80454-g.
A technique was developed for transfer of fat and polychlorinated biphenyls from cod liver oil into the lipophilic gel Lipidex 5000. Subsequent elution of the gel separated about 60% of the fat from the sample. Following further purification on aluminium oxide and silica gel, toxic non-ortho- and mono-ortho-PCB congeners were isolated in two separate fractions on charcoal. Recoveries were studied by addition of twelve different PCB congeners to 0.2 g of fat. The non-ortho-PCBs were labelled with 13C. The recoveries of 5-50 ng of the unlabelled compounds were 80-100% and those of 50-100 pg of the labelled compounds were 76-106%.
开发了一种将脂肪和多氯联苯从鳕鱼肝油转移到亲脂性凝胶Lipidex 5000中的技术。随后对凝胶进行洗脱，从样品中分离出约60%的脂肪。在氧化铝和硅胶上进一步纯化后，有毒的非邻位和单邻位多氯联苯同系物在木炭上被分离成两个单独的馏分。通过向0.2 g脂肪中添加十二种不同的多氯联苯同系物来研究回收率。非邻位多氯联苯用13C标记。5 - 50 ng未标记化合物的回收率为80 - 100%，50 - 100 pg标记化合物的回收率为76 - 106%。
