Marucci L, Baroni G S, Mancini R, Benedetti A, Jezequel A M, Orlandi F
Department of Gastroenterology, University of Ancona, Italy.
J Hepatol. 1993 Feb;17(2):163-9. doi: 10.1016/s0168-8278(05)80032-7.
The aim of the present investigation was to conduct an immunohistochemical study using bromodeoxyuridine (BrdU) incorporation as a marker of S-phase cells and cytokeratins as markers of biliary epithelial cells, in bile-duct-ligated rats at intervals of 1, 3, 7, 14 and 21 days after total biliary obstruction. Data obtained using only BrdU incorporation by S-phase nuclei were compared with those obtained by the simultaneous demonstration of S-phase nuclei and cytoplasmic cytokeratins. The labelling index of parenchymal liver cells and of biliary epithelial cells was evaluated as an index of the cellular growth pattern after total biliary obstruction. Our data show that following total biliary obstruction: (a) cell proliferation follows a similar pattern for biliary epithelial cells hepatocytes with a peak on the 3rd day; (b) the labelling index is significantly higher in biliary epithelial cells than in hepatocytes; and (c) sequential immunohistochemical staining using cytokeratin as a marker allows better identification of biliary epithelial cells, especially when the ductular lumen is not clearly outlined, or in isolated biliary cells which appear as components of the wall of the ducts of Hering.
本研究的目的是在胆总管结扎的大鼠中，于完全性胆管梗阻后1天、3天、7天、14天和21天的时间间隔，进行一项免疫组织化学研究，使用溴脱氧尿苷（BrdU）掺入作为S期细胞的标志物，细胞角蛋白作为胆管上皮细胞的标志物。将仅通过S期细胞核的BrdU掺入获得的数据，与通过同时显示S期细胞核和细胞质细胞角蛋白获得的数据进行比较。将实质肝细胞和胆管上皮细胞的标记指数评估为完全性胆管梗阻后细胞生长模式的指标。我们的数据显示，在完全性胆管梗阻后：（a）胆管上皮细胞和肝细胞的细胞增殖模式相似，在第3天达到峰值；（b）胆管上皮细胞的标记指数显著高于肝细胞；（c）使用细胞角蛋白作为标志物进行连续免疫组织化学染色，可以更好地识别胆管上皮细胞，特别是当胆小管腔轮廓不清晰时，或在作为赫林管管壁成分出现的孤立胆管细胞中。
