Engh E, Clausen O P, Purvis K, Stien R
Andrology Laboratory, National Hospital, Oslo, Norway.
Paraplegia. 1993 Jan;31(1):3-12. doi: 10.1038/sc.1993.2.
Semen was obtained by vibration-induced ejaculation from 5 spinal injured men once a week for 5 consecutive weeks under standardised conditions. The site of the spinal lesions varied from C5 to Th10. Although in all subjects except one, the total sperm count in the first ejaculate was within normal limits, conventional criteria indicated a high degree of asthenoteratozoospermia in all cases. In subsequent ejaculates there was no major general improvement in motility, vitality or morphology. However, 2 individuals exhibited a marked increase in the proportion of motile sperm in the ejaculate over the next 3 weeks. Flow cytometry of the same sperm samples indicated a high degree of abnormal chromatin condensation and reduced binding of a fluorescent acrosomal marker in the first ejaculates. No improvement in these parameters could be detected with time. Assessment of accessory sex gland function using specific secretory markers indicated that compared to the normal population, the vesicular contribution was markedly reduced in 3 subjects and prostatic contribution in the 2 remaining subjects in the first and subsequent ejaculations. Ejaculate volumes were consistently low in all subjects during the observation period. In contrast, total epididymal secretion was comparable to normal ejaculates. Prostatic contributions to the ejaculate increased significantly over the first 4 weeks. In conclusion, regular vibration-induced ejaculation at weekly intervals could not improve sperm quality in paraplegic men to an acceptable degree for assisted fertilisation to be recommended. Although certain aspects of sperm quality, as judged by conventional criteria, were improved in some cases, flow cytometry revealed persistent chromatin and acrosomal abnormalities.
在标准化条件下，通过振动诱导射精，每周从5名脊髓损伤男性中获取精液，连续进行5周。脊髓损伤部位从C5到Th10不等。除1名受试者外，所有受试者首次射精的精子总数均在正常范围内，但按照传统标准，所有病例均显示出高度的弱畸精子症。在随后的射精中，精子活力、存活率或形态学方面没有总体上的显著改善。然而，有2名个体在接下来的3周内，射精中活动精子的比例显著增加。对相同精子样本进行流式细胞术分析表明，首次射精时精子染色质浓缩高度异常，顶体荧光标记物的结合减少。随着时间推移，这些参数未见改善。使用特定分泌标记物评估附属性腺功能表明，与正常人群相比，在首次射精及随后射精中，3名受试者的精囊分泌物显著减少，其余2名受试者的前列腺分泌物减少。在观察期内，所有受试者的射精量一直很低。相比之下，附睾总分泌量与正常射精量相当。前列腺对射精的贡献在最初4周内显著增加。总之，对于截瘫男性，每周定期进行振动诱导射精并不能将精子质量提高到推荐辅助受精的可接受程度。尽管按照传统标准判断，某些精子质量方面在某些情况下有所改善，但流式细胞术显示染色质和顶体持续存在异常。
