5-羟甲基-2'-脱氧尿苷和3-氨基苯甲酰胺对培养的人淋巴细胞染色体畸变的影响。

Effects of 5-hydroxymethyl-2'-deoxyuridine and 3-aminobenzamide on chromosome aberrations in cultured human lymphocytes.

作者信息

Zhang A, Lin M S, Fujimoto A

机构信息

Department of Pediatrics, Los Angeles County/University of Southern California Medical Center 90033.

出版信息

Mutat Res. 1993 Apr;301(4):229-33. doi: 10.1016/0165-7992(93)90062-z.

DOI:10.1016/0165-7992(93)90062-z

PMID:7680756

Abstract

Effects of 5-hydroxymethyl-2'-deoxyuridine (HmdUrd, a thymidine analog) and 3-aminobenzamide (3AB) on chromosome aberrations in cultured human lymphocytes were studied. The results show that HmdUrd is an effective clastogen in human peripheral lymphocytes. When cells were treated with HmdUrd and 3AB, a synergistic effect on chromatid gaps, breaks and exchanges was found. These findings support the hypotheses that 5-hydroxymethyluracil (HmUra) residues in DNA are formed and then removed by the action of 5-HmUra-DNA glycosylase (Teeber et al., 1984) and that 3AB interferes with the completion of DNA repair following HmUra excision.

摘要

研究了5-羟甲基-2'-脱氧尿苷（HmdUrd，一种胸腺嘧啶类似物）和3-氨基苯甲酰胺（3AB）对培养的人淋巴细胞染色体畸变的影响。结果表明，HmdUrd是人类外周淋巴细胞中的一种有效断裂剂。当细胞用HmdUrd和3AB处理时，发现对染色单体间隙、断裂和交换有协同作用。这些发现支持以下假设：DNA中的5-羟甲基尿嘧啶（HmUra）残基形成后通过5-HmUra-DNA糖基化酶的作用被去除（Teeber等人，1984年），并且3AB在HmUra切除后干扰DNA修复的完成。