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从全血中分离RNA——基于RNA诊断的开端？

Isolating RNA from whole blood--the dawn of RNA-based diagnosis?

作者信息

Macfarlane D E, Dahle C E

机构信息

Department of Internal Medicine, University of Iowa, Iowa City 52242.

出版信息

Nature. 1993 Mar 11;362(6416):186-8. doi: 10.1038/362186a0.

DOI:10.1038/362186a0
PMID:7680772
Abstract

A novel cationic surfactant solution is used to lyse blood cells and precipitate RNA and DNA. The RNA is recovered by extracting the pellet with a small volume of guanidinium isothiocyanate or formamide.

摘要

一种新型阳离子表面活性剂溶液用于裂解血细胞并沉淀RNA和DNA。通过用少量异硫氰酸胍或甲酰胺提取沉淀来回收RNA。

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