Petruzzelli G J, Snyderman C H, Johnson J T, Myers E N
Department of Otolaryngology, Eye and Ear Institute of Pittsburgh, PA 15213.
Ann Otol Rhinol Laryngol. 1993 Mar;102(3 Pt 1):215-21. doi: 10.1177/000348949310200309.
The angiogenic potential of head and neck tumors compared to nonneoplastic control tissues was investigated by using the chick embryo chorioallantoic membrane (CAM) as a bioassay for angiogenesis. Eighty tumor specimens from 10 patients with squamous cell carcinoma of the head and neck were grafted onto the CAM of 7-day chick embryos. The presence of tumor in the original explant was confirmed histologically. Forty-four embryos (55%) survived and were evaluated histologically at day 17. Tumors were growing on or invading the CAM mesoderm in 30 of 44 embryos (68%). Before harvesting of the membranes, the tumors and surrounding blood vessels were photographed, and the angiogenic responses were graded by a panel of blinded observers. Tumor explants elicited a significantly greater angiogenic response than nontumor controls (p = .01). We conclude that head and neck squamous cell carcinomas can induce an angiogenic response in vivo, presumably secondary to the production of an unidentified angiogenic factor, and that the chick embryo CAM is an effective model for quantifying angiogenesis induced by head and neck tumors.
通过使用鸡胚绒毛尿囊膜（CAM）作为血管生成的生物测定法，研究了头颈部肿瘤与非肿瘤对照组织相比的血管生成潜力。将来自10名头颈部鳞状细胞癌患者的80个肿瘤标本移植到7日龄鸡胚的CAM上。通过组织学确认原代外植体中存在肿瘤。44个胚胎（55%）存活并在第17天进行了组织学评估。44个胚胎中有30个（68%）的肿瘤生长在或侵入了CAM中胚层。在收获膜之前，对肿瘤和周围血管进行拍照，并由一组不知情的观察者对血管生成反应进行分级。肿瘤外植体引发的血管生成反应明显大于非肿瘤对照（p = 0.01）。我们得出结论，头颈部鳞状细胞癌可在体内诱导血管生成反应，推测是由于产生了一种未确定的血管生成因子，并且鸡胚CAM是量化头颈部肿瘤诱导的血管生成的有效模型。
