Yamashita T, Kasai N, Miyoshi I, Sasaki N, Maki K, Sakai M, Nishi S, Namioka S
Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Biochem Biophys Res Commun. 1993 Mar 15;191(2):715-20. doi: 10.1006/bbrc.1993.1276.
Transgenic mice were produced by the microinjection of the cDNA of human alpha-fetoprotein (AFP) under control of the enhancer/promoter of the human beta-actin gene. Among the 4 mouse lines where the transgene were stable transmitted to the progeny, 3 produced human AFP. The expression was not developmental stage-specific nor tissue-specific as predicted from the properties of enhancer/promoter. The serum human AFP levels of adult mice were 30-600-fold higher than those of mouse AFP. These mice could be useful for the studies of possible biological functions of AFP during development as well as in malignancies.
