Faye L, Gomord V, Fitchette-Lainé A C, Chrispeels M J
European Institute for Peptide Research, LTI-CNRS URA 203, University of Rouen, Mont Saint Aignan, France.
Anal Biochem. 1993 Feb 15;209(1):104-8. doi: 10.1006/abio.1993.1088.
Antisera raised against the plant glycoproteins beta-fructosidase and horseradish peroxidase can be fractionated on an affinity column of honeybee venom phospholipase A2 to produce serum fractions that are specific for either the alpha 1-->3 fucose or beta 1-->2 xylose epitopes commonly found on the Asn-linked glycans of plant glycoproteins. This affinity purification strategy relies on the absence of beta 1-->2 xylose from the glycan of the venom protein. Such antibody preparations can be used for the detection of these sugar epitopes on glycoproteins.
针对植物糖蛋白β-果糖苷酶和辣根过氧化物酶产生的抗血清，可在蜜蜂毒液磷脂酶A2亲和柱上进行分离，以产生对植物糖蛋白天冬酰胺连接聚糖上常见的α1→3岩藻糖或β1→2木糖表位具有特异性的血清组分。这种亲和纯化策略依赖于毒液蛋白聚糖中不存在β1→2木糖。此类抗体制剂可用于检测糖蛋白上的这些糖表位。
