Wood E R, Berger H, Sherman P A, Lapetina E G
Division of Cell Biology, Burroughs Wellcome Co., Research Triangle Park, North Carolina 27709.
Biochem Biophys Res Commun. 1993 Mar 31;191(3):767-74. doi: 10.1006/bbrc.1993.1283.
Polymerase chain reaction amplification of reverse transcribed RNA (RNA-PCR) was used to isolate overlapping cDNA clones encoding an inducible form of nitric oxide synthase (NOS) from purified rat hepatocytes. NOS gene expression in hepatocytes is dependent upon bacterial lipopolysaccharide (LPS) and cytokine treatment. The predicted amino acid sequence of rat hepatocyte inducible NOS is 94% identical with that from the mouse macrophage cell line, RAW264.7. Differences between the two sequences occur as sporadic amino acid substitutions with no major gaps or insertions. These differences are most likely the result of species variability which suggests that hepatocytes and macrophages express the same NOS gene upon induction.
采用逆转录RNA的聚合酶链反应扩增法（RNA-PCR），从纯化的大鼠肝细胞中分离出编码诱导型一氧化氮合酶（NOS）的重叠cDNA克隆。肝细胞中的NOS基因表达取决于细菌脂多糖（LPS）和细胞因子处理。大鼠肝细胞诱导型NOS的预测氨基酸序列与小鼠巨噬细胞系RAW264.7的序列有94%的同源性。两个序列之间的差异表现为散在的氨基酸替换，没有大的缺口或插入。这些差异很可能是物种变异性的结果，这表明肝细胞和巨噬细胞在诱导后表达相同的NOS基因。
