降钙素基因相关肽对炎性水肿的调节作用

The modulation of inflammatory oedema by calcitonin gene-related peptide.

作者信息

Newbold P, Brain S D

机构信息

Biomedical Sciences Division, King's College, London.

出版信息

Br J Pharmacol. 1993 Mar;108(3):705-10. doi: 10.1111/j.1476-5381.1993.tb12865.x.

DOI:10.1111/j.1476-5381.1993.tb12865.x

PMID:7682134

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1908016/

Abstract

The effect of calcitonin gene-related peptide (CGRP) when given with or as a pretreatment to oedema inducing agents was investigated in the skin and paws of male Wistar and Sprague Dawley rats. 2. Oedema formation at intradermally-injected sites in the skin was measured by a 125I-labelled human serum albumin accumulation technique and paw oedema was measured by a weight displacement technique. 3. CGRP (30 pmol) when given with, or as a 20 min pretreatment, markedly potentiated oedema formation induced by substance P (100 pmol) in rat skin. In comparison, CGRP had little effect on 5-hydroxytryptamine (5-HT, 0.1-3 nmol)-induced oedema when given as a co-injection but significantly potentiated 5-HT-induced oedema when given as a 20 min pretreatment in the skin. Similar results were obtained in both Wistar and Sprague Dawley rats. 4. Pretreatment with CGRP (30 pmol) had little modulatory effect on oedema induced by substance P (100 pmol) in the presence of the vasodilator prostanoid, prostaglandin E1 (PGE1, 850 pmol) in the skin of Wistar rats. 5. Pretreatment with CGRP (30 pmol) caused a non-significant increase in carrageenin (300 micrograms)-induced oedema in the hind paw of Wistar rats. Capsaicin (100 nmol) given as a pretreatment had little effect on carrageenin-induced oedema. 6. CGRP (30 pmol), given as a pretreatment, had little modulatory effect on 5-HT (3 nmol)-induced oedema in the paw of Wistar rats but a non-significant decrease in paw oedema was observed in Sprague Dawley rats. 7. The results suggest that CGRP, either given together with .or as a pretreatment to mediators of increased microvascular permeability, depending on circumstance, can act in a synergistic manner to increase oedema formation. Little evidence was obtained for an anti-inflammatory effect of CGRP in either the rat skin or paw.

摘要

在雄性Wistar和Sprague Dawley大鼠的皮肤和爪子上，研究了降钙素基因相关肽（CGRP）与致水肿剂同时给药或作为预处理给药时的作用。2. 通过125I标记的人血清白蛋白蓄积技术测量皮肤皮内注射部位的水肿形成，并用重量位移技术测量爪部水肿。3. CGRP（30 pmol）与P物质（100 pmol）同时给药或作为20分钟预处理给药时，可显著增强大鼠皮肤中P物质诱导的水肿形成。相比之下，CGRP作为共注射给药时对5-羟色胺（5-HT，0.1 - 3 nmol）诱导的水肿影响不大，但在皮肤中作为20分钟预处理给药时可显著增强5-HT诱导的水肿。在Wistar和Sprague Dawley大鼠中均获得了类似结果。4. 在Wistar大鼠皮肤中，在存在血管扩张性前列腺素E1（PGE1，850 pmol）的情况下，CGRP（30 pmol）预处理对P物质（100 pmol）诱导的水肿几乎没有调节作用。5. CGRP（30 pmol）预处理使Wistar大鼠后爪角叉菜胶（300微克）诱导的水肿有不显著增加。辣椒素（100 nmol）作为预处理对角叉菜胶诱导的水肿影响不大。6. CGRP（30 pmol）作为预处理对Wistar大鼠爪部5-HT（3 nmol）诱导的水肿几乎没有调节作用，但在Sprague Dawley大鼠中观察到爪部水肿有不显著降低。7. 结果表明，CGRP与微血管通透性增加的介质同时给药或作为预处理给药时，根据情况可协同作用以增加水肿形成。在大鼠皮肤或爪子中几乎没有获得CGRP具有抗炎作用的证据。